Protein Info for GFF2343 in Variovorax sp. SCN45

Annotation: ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 27 to 48 (22 residues), see Phobius details amino acids 89 to 113 (25 residues), see Phobius details amino acids 132 to 160 (29 residues), see Phobius details amino acids 206 to 231 (26 residues), see Phobius details amino acids 252 to 275 (24 residues), see Phobius details PF12911: OppC_N" amino acids 17 to 64 (48 residues), 30.6 bits, see alignment 2.5e-11 PF00528: BPD_transp_1" amino acids 105 to 288 (184 residues), 115.1 bits, see alignment E=3.2e-37

Best Hits

Swiss-Prot: 43% identical to GSID_SHIFL: Glutathione transport system permease protein GsiD (gsiD) from Shigella flexneri

KEGG orthology group: K02034, peptide/nickel transport system permease protein (inferred from 95% identity to vpe:Varpa_5004)

MetaCyc: 43% identical to glutathione ABC transporter membrane subunit GsiD (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Dipeptide transport system permease protein DppC (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (291 amino acids)

>GFF2343 ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines) (Variovorax sp. SCN45)
MSSITAPSAAALKVPGFWRRALHHRSFVLGGILTLLLLLAAAVSLVWTPWSPYDMDMANK
LKPPMASHWLGTDTYGRDVASLLLVGARASILVGVIAVGIGLVVGTALGLLAAARRGWVE
EAIMRLTDFSLAFPAILSAIMLTAVFGAGIVNAIVAIGIYNIPTFARITRASANAIWSRE
YVAAARACGKGSFAITMQHVLPNISAVLIVQITIRFAIAILAEAALSYLGLGTQPPQPSW
GRMLSEAQTMMFQSPLLAVFPGMAIAFAVLGLNLLGDGLRDLLDPRLARAR