Protein Info for GFF2330 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 PF01494: FAD_binding_3" amino acids 16 to 355 (340 residues), 215.1 bits, see alignment E=8.7e-68

Best Hits

KEGG orthology group: K05712, 3-(3-hydroxy-phenyl)propionate hydroxylase [EC: 1.14.13.-] (inferred from 58% identity to bge:BC1002_5806)

Predicted SEED Role

"3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-)" in subsystem Cinnamic Acid Degradation (EC 1.14.13.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-

Use Curated BLAST to search for 1.14.13.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>GFF2330 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
LKVENDAAFGGDALHDVAIIGLGPCGAALANLLGQQGLKVLVLEKEADIYRLPRAIHFDG
ECMRAFQAMGIADDLYPDLLVSPGMKFVDAAGRVLIDYTRPQDLGPQGWHPSYRFHQPQL
EETLRKRLRDHANVEVRLRHDLFAIEPQDDRVVLRVEDMRSGRLLRTAARYVVGCDGARS
LVRRFMGTELDDMHSHERWLVLDVLLHGERPDLGDHSIQYCDPARPATYVRGVGKRRRWE
LMLMPGDDPVAMVRPETVWPLLSRWVAPHEATLERPAVYTFHSVVARGWRRDRLLIAGDA
AHQTPPFMGQGMCAGIRDAFNLAWKLAAVVKGQLPTRLLDTYESERSPHVREFIETAVRL
GGVIQATDPDVARERDARMAASPLVIKTPQPALGPGWHAGGAVAGRVAPQPMLDDGRRFD
DGLGDRFALVATPEALGTCAALASERLVLREAGDGAVKQWLHDAGCEALLLRPDRHIAAS
AAHAADIPAMVRQFVTGEGLA