Protein Info for GFF233 in Sphingobium sp. HT1-2

Annotation: 'Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II' transl_table=11

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 PF13328: HD_4" amino acids 26 to 176 (151 residues), 164.6 bits, see alignment E=6e-52 TIGR00691: RelA/SpoT family protein" amino acids 26 to 698 (673 residues), 682.9 bits, see alignment E=2.5e-209 PF01966: HD" amino acids 45 to 144 (100 residues), 38.5 bits, see alignment E=5.3e-13 PF04607: RelA_SpoT" amino acids 236 to 346 (111 residues), 145.5 bits, see alignment E=3.2e-46 PF02824: TGS" amino acids 385 to 444 (60 residues), 79.9 bits, see alignment 4.6e-26 PF19296: RelA_AH_RIS" amino acids 456 to 547 (92 residues), 47 bits, see alignment E=1.3e-15 amino acids 554 to 612 (59 residues), 30.2 bits, see alignment 1.8e-10 PF13291: ACT_4" amino acids 627 to 700 (74 residues), 50.7 bits, see alignment E=9.3e-17 PF01842: ACT" amino acids 629 to 692 (64 residues), 27.3 bits, see alignment E=1e-09 PF22629: ACT_AHAS_ss" amino acids 630 to 697 (68 residues), 32.6 bits, see alignment E=3e-11

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 94% identity to sch:Sphch_2797)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (701 amino acids)

>GFF233 'Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II' transl_table=11 (Sphingobium sp. HT1-2)
MLRQYELVERVKRYDPDADEAMINRAYVFSVQKHGSQKRASGDPYFSHPIEVAGILTDFY
LDDQTIVTALLHDTIEDTLVTYDEIEEAFGKDVARMVDGVTKLSKIEAMSENERAAENLR
KFLLAMSDDIRVLLVKLADRLHNMRTLHFIKNEQKRRRIAKETMDIYAPLAERIGMYDFM
REMQLLSFRVLEPEAYDSITKRLEQLKEGGHDKVDRIGAELQLLLGGHNLSVTVSGREKH
PYSIWKKMQERHISFEQLTDVMAFRVITETTEDCYRALGVIHQTFKMVPGRFKDYISTPK
RNGYRSLHTTVIHQENARIEVQIRSRDMHNDAELGLAAHWAYKQKGDATDQNAAWLRDLV
EILEQSQDADELLEHTRMAMYQDRIFAFSPKGELHQLPKGSTPVDFAYAVHTSLGNQTVG
AKVNGRVVPLRTSLENGDQVEILKSAGQEPQPGWLSFAITGKARAAIRRFIRQKQRGEEV
ALGEKLYEEIIGRFSPELASELGDKALNAALKRLKLEDRASLMVAIATHRLLDSEVMEAL
IPGSTSGQELEEAHPRQHAPVSIRGLTPGIAYKLSECCHPVPGDRIVGIRRTGEPIEVHT
IDCRTLEAGQDDDWVDLSWDSKSKGGTARLQVIVKNQPGALAAVANVFGATKANILNLQL
VNREGPFHTDIIDLEVADAQHLSRIVSALRALDTVVQADRV