Protein Info for GFF2321 in Xanthobacter sp. DMC5

Annotation: ECF RNA polymerase sigma factor EcfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 197 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 18 to 169 (152 residues), 104 bits, see alignment E=3.2e-34 PF22029: PhyR_sigma2" amino acids 25 to 76 (52 residues), 53.8 bits, see alignment E=3.9e-18 PF04542: Sigma70_r2" amino acids 25 to 89 (65 residues), 71.5 bits, see alignment E=9.9e-24 PF07638: Sigma70_ECF" amino acids 37 to 167 (131 residues), 25.8 bits, see alignment E=2.2e-09 PF08281: Sigma70_r4_2" amino acids 116 to 167 (52 residues), 56.5 bits, see alignment E=4.2e-19 PF04545: Sigma70_r4" amino acids 120 to 167 (48 residues), 32.3 bits, see alignment E=1.4e-11

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 96% identity to xau:Xaut_2021)

Predicted SEED Role

"RNA polymerase sigma factor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (197 amino acids)

>GFF2321 ECF RNA polymerase sigma factor EcfG (Xanthobacter sp. DMC5)
MDLPAPSERTRTEMDAAALRTQVLEFLPAVRAFARSLTRNRTEADDLVQETLLKALSNMD
KFDPGTNLRAWLFTILRNTYYTEIRKRRRENDGMAALAQQDSNIGPSQEWSATLTSLKDA
LALLPDDQREALVLVGAAGLSYEEAAEVCGCALGTIKSRVNRARAKLLVLMGAEVTADVV
EGDAVAMSAARSSLSTS