Protein Info for GFF2307 in Xanthobacter sp. DMC5

Annotation: 3-dehydro-bile acid delta(4,6)-reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF03486: HI0933_like" amino acids 7 to 399 (393 residues), 198.6 bits, see alignment E=4.1e-62 PF07992: Pyr_redox_2" amino acids 7 to 173 (167 residues), 29 bits, see alignment E=2.5e-10 TIGR00275: flavoprotein, HI0933 family" amino acids 8 to 398 (391 residues), 242.4 bits, see alignment E=7.4e-76 PF01494: FAD_binding_3" amino acids 8 to 43 (36 residues), 25 bits, see alignment 3.7e-09 PF13450: NAD_binding_8" amino acids 10 to 39 (30 residues), 27 bits, see alignment (E = 1.5e-09) TIGR03862: flavoprotein, TIGR03862 family" amino acids 28 to 403 (376 residues), 448 bits, see alignment E=2.3e-138 PF22780: HI0933_like_1st" amino acids 194 to 343 (150 residues), 77.7 bits, see alignment E=3.4e-25

Best Hits

KEGG orthology group: K07007, (no description) (inferred from 71% identity to xau:Xaut_1928)

Predicted SEED Role

"NAD(FAD)-utilizing dehydrogenases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (407 amino acids)

>GFF2307 3-dehydro-bile acid delta(4,6)-reductase (Xanthobacter sp. DMC5)
VSPSSRSIAIVGAGPAGLAAAEVLSAAGFRVALYDRMPSAARKFLMAGRGGLNLTHSEPA
DAFRARYGTAAERLAPALAAFPPQALRDWAAGLGEETFVGSSGRVFPRSFKASPLLRAWL
GRLAANGVTFHPRHDWRGFDAASALLFETPDGPAEVKADAVILALGGASWPRLGSDGGFV
PALRALGVEVKDLVPANMGVLVPWSEVFRQKFAGTPLKRIALTFNGVTVKGEAMVSATGL
EGGAIYALSAPLRRALADGPVTLHVDLRPDLDGAELARRLGGGPGSASLSTLLRKAAGLA
PVAVGLIREATGPARPDPVTLARLVKALPVTITGHAGLERAISSAGGIGWDGIGPDFSLR
GLPPHLPPVFVAGEMLDWEAPTGGYLLQAVIATGRAAGAGALARLSR