Protein Info for GFF227 in Variovorax sp. SCN45

Annotation: ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 346 transmembrane" amino acids 23 to 44 (22 residues), see Phobius details amino acids 144 to 173 (30 residues), see Phobius details amino acids 193 to 222 (30 residues), see Phobius details amino acids 265 to 290 (26 residues), see Phobius details amino acids 310 to 332 (23 residues), see Phobius details PF12911: OppC_N" amino acids 9 to 47 (39 residues), 39.3 bits, see alignment 4.7e-14 PF00528: BPD_transp_1" amino acids 162 to 343 (182 residues), 110.2 bits, see alignment E=1e-35

Best Hits

Swiss-Prot: 52% identical to YEJE_ECOLI: Inner membrane ABC transporter permease protein YejE (yejE) from Escherichia coli (strain K12)

KEGG orthology group: K13895, microcin C transport system permease protein (inferred from 91% identity to vpe:Varpa_2844)

MetaCyc: 52% identical to putative oligopeptide ABC transporter membrane subunit YejE (Escherichia coli K-12 substr. MG1655)
3.6.3.23-RXN [EC: 7.4.2.6]

Predicted SEED Role

"Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (346 amino acids)

>GFF227 ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines) (Variovorax sp. SCN45)
MVSGSTHISPGRRAWLRFKRNRLGFWSLVIFTVMVVLSLFAEVLSTDKPLVLRYEGRTYF
PVLRDYSEKTFGGDFETPADYLDPYIRDRITQGDNWAIYAPNPYGPKTLNYFAKAPSPAA
PTADNIFGTDDRGRDLLAQLIYGFRVSVLFGLALTAIGTVLGVAAGAVQGYFAGKVDLAF
QRFIEIWSSMPELYLLIIFSAIFAPSVALLLILLSLFGWMGLADYVRAEFLRNRQMDYVR
AARALGVSNLQIMWRHILPNSMVPVVTFLPFRMSAAILALTSLDFLGLGVPPGTPSLGEL
LSQGKANIDAWWISISTFGVLVVTLMLLTFMGDALRDALDPRKADK