Protein Info for GFF227 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: Homolog of E. coli HemX protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to HEMX_ECOLI: Protein HemX (hemX) from Escherichia coli (strain K12)
KEGG orthology group: K02496, uroporphyrin-III C-methyltransferase [EC: 2.1.1.107] (inferred from 99% identity to seh:SeHA_C4266)Predicted SEED Role
"Homolog of E. coli HemX protein"
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (31/36 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (14/15 steps found)
- siroheme biosynthesis (4/4 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (10/13 steps found)
- factor 430 biosynthesis (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.107
Use Curated BLAST to search for 2.1.1.107
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (389 amino acids)
>GFF227 Homolog of E. coli HemX protein (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MTEQEKSSAVVDETRESVETTPQPVNTEKKSKNGAALVLSAVAIAIALAAGIGLYGWGKQ QATAQTETSDALATQLTALQKAQESQKAELEGIIKKQAAQLDDANRQQAALAKQLDEVQQ KVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAALLKSADASLADMNDPSLITA RRAITDDIASLSSVAQIDYDGIILKLNQLSNQIDNLRLADNDTDGSPMDSDSSELSSSLS EWRVNLQKSWQNFMDSFITIRRRDDTAVPLLAPNQDVYLRENIRSRLLVAAQAVPRHQEE TYRQALDNVSTWVRAYYDTDDAATKAFLEEVDKLSQQNITMDLPETLGSQAILEKLMQTR VRNLLAQPTVSTAAPATQTDAPAAAPQGE