Protein Info for GFF2255 in Pseudomonas sp. DMC3

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1243 PF00289: Biotin_carb_N" amino acids 33 to 142 (110 residues), 140 bits, see alignment E=1.9e-44 TIGR02712: urea carboxylase" amino acids 34 to 1241 (1208 residues), 1886.2 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 148 to 351 (204 residues), 200.8 bits, see alignment E=8.9e-63 PF07478: Dala_Dala_lig_C" amino acids 173 to 323 (151 residues), 42.2 bits, see alignment E=3e-14 PF02785: Biotin_carb_C" amino acids 370 to 478 (109 residues), 104 bits, see alignment E=2.1e-33 TIGR00724: biotin-dependent carboxylase uncharacterized domain" amino acids 487 to 792 (306 residues), 285.7 bits, see alignment E=4.1e-89 PF02626: CT_A_B" amino acids 510 to 782 (273 residues), 316.3 bits, see alignment E=8.3e-98 PF02682: CT_C_D" amino acids 846 to 1064 (219 residues), 138.8 bits, see alignment E=9e-44 PF00364: Biotin_lipoyl" amino acids 1181 to 1240 (60 residues), 53.5 bits, see alignment 7.6e-18

Best Hits

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6)" in subsystem Urea decomposition (EC 6.3.4.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1243 amino acids)

>GFF2255 Carbamoyl-phosphate synthase large chain (Pseudomonas sp. DMC3)
VGLSEVCFFHPGRPGCLSKNSGTARSPFRGYAMFEKILIANRGAIACRILRTINVLKAQG
VAVYSEADAASLHISRAAEAHSLGEGAASATYLAVDKILTIAKQSGATAIHPGYGFLSEN
AAFAEACEAQGIAFIGPTPEQLRVFGLKHTARALAKQHGVPMLEGTDLLDSLDCALSAAE
QVGYPVMLKSTAGGGGIGMRVCRSASELSESFEAVKRLGQNNFSDAGVFIEKYIERARHL
EVQVFGDGCGEVVALGVRDCSVQRRNQKVLEETPAPNLPEGMADELCAAAIKLAQAVNYR
SAGTVEFVFDSEAQRFYFLEVNTRLQVEHGVTEQVWDVDLVRWMIELAAGDLPPLNELYQ
GLEPQGHAIQARLYAEDPGRDFQPSPGLLTAVQFPPANGVELRIDTWVEAGVEIPPDFDP
MIAKLIAWAPSREQARAELHQALGDSLLYGVETNRDYLRQILLDAPFASGQPWTRCLENL
LYHANTFEVLSAGTQTSVQDYPGRLGYWAVGVPPSGPMDSRALRLGNRLLGNEQGAAALE
ITMSGPILRFNCDAVVAVTGAPIALTLDAEPVPMNTALLIPAGATLQLGSISGAGARSYL
CLRGGLQVPDYLGSKSTFTLGQCGGHGGRALRAGDVLHVPALTERSVGQQLAEQHITALP
SVRQIRVIYGPHGAPEYFSENYIATFFATQWEVHFNSSRTGVRLIGPKPEWVREDGGEAG
LHPSNIHDNPYAIGAVDFTGDMPVILGPDGPSLGGFVCPVTVIEADLWQLGQLKAGDKIQ
FIPVDLKTARDLALKWDLPCGSWLASDGARSDTAATSSLASQLPQGIVSPVVLELGQGDR
RLVGRVSGDTHLLLEIGAPELDLVLRFRAHALMQALESQALHGVIDLTPGIRSLQVHYQP
EQLPLADLLGIIAGEWDAVCAAKDLQVPSRIVHLPLSWDDPACQLAIEKYMTTVRKDAPW
CPSNLEFIRRINDLPNLDEVQRTVFDASYLVMGLGDVYLGAPVATPLDPRHRLVTTKYNP
ARTWTAENSVGIGGAYMCVYGMEGPGGYQFVGRTLQMWNRYREVAAFDGKPWLLRFFDQI
RFYPVSAEELLRIRRDFPLGRFDLNIEHSQLNLADYQAFLSQEAEGISAFRAQQQGAFKA
ERERWIASGQAHFDSEEAVPDASEEAPLESGEHSVDSHIAGNLWQVQVEAGQRVAAGDVL
VILESMKMEIPVLAPLAGVVREIRVQPGSAVRAGQRVAVLELD