Protein Info for GFF2253 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1016 TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 2 to 1015 (1014 residues), 1563.2 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 44 to 103 (60 residues), 56.7 bits, see alignment 2.8e-19 PF00384: Molybdopterin" amino acids 107 to 589 (483 residues), 99.2 bits, see alignment E=3.9e-32 PF01568: Molydop_binding" amino acids 894 to 1009 (116 residues), 61.8 bits, see alignment E=9.3e-21

Best Hits

Swiss-Prot: 94% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 70% identity to avn:Avin_03810)

MetaCyc: 94% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1016 amino acids)

>GFF2253 Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MQVSRRQFFKICAGGMAGTTAAALGFAPGVALAETRQYKLLRTRETRNTCTYCSVGCGLL
MYSLGDGAKNAKASIFHIEGDPDHPVSRGALCPKGAGLVDFIHSESRLKFPQYRAPGSDK
WQQISWEEAFDRIAKLMKEDRDANYQAQNAEGVTVNRWLTTGMLCASASSNETGYLTQKF
SRALGMLAVDNQARV*HGPTVASLAPTFGRGAMTNHWVDIKNANLVVVMGGNAAEAHPVG
FRWAMEAKIHNGAKLIVIDPRFTRTASVADFYAPIRSGTDIAFLSGVMLYLLTNEKYNRE
YTEAYTNASLIVREDFGFDDGLFTGYDADKRQYDKTSWHYELDENGFAKHDTTLQHPRCV
WNLLKQHVSRYTPDMVENICGTPKADFLKVCEYIAETSAKDKTASFLYALGWTQHSIGAQ
NIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSQSLPGYLTLPSEKQTDLQT
YLAANTPKPLLKDQVNYWGNYPKFFVSMMKAFFGDKATAENSWGFDWLPKWDKGYDVLQY
FEMMKQGKVNGYICQGFNPVASFPNKNKVVASLSKLKYLVTIDPLNTETSTFWQNHGESN
DVDPAKIQTEVFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGIAMTDGEILAGIFLRL
RKMYSEQGGANPEQVLNMTWNYTKPYEPASEEVAMESNGKALADLIDPATGAVVVKKGQQ
LSSFAQLRDDGTTSSGCWIFAGSWTPEGNMMARRDNADPSGLGNTLGWAWAWPLNRRILY
NRASADPQGNPWDPKRQLLKWEGGKWAGWDIPDYSAAAPGSDVGPFIMQPEGMGRLFAID
KMAEGPFPEHYEPFETPLGTNPLHPNVISNPAARIFKDDADALGKADKFPYVGTTYRLTE
HFHYWTKHALLNAIAQPEQFVEIGEKLANKLGIAHGDTVKVSSNRGYIKAKAVVTKRIRT
LKADGKDIDTIGIPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV