Protein Info for GFF222 in Variovorax sp. SCN45

Annotation: Translation initiation factor 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 993 PF04760: IF2_N" amino acids 1 to 52 (52 residues), 37.6 bits, see alignment 6.1e-13 amino acids 416 to 466 (51 residues), 60.6 bits, see alignment 4.1e-20 PF08364: IF2_assoc" amino acids 61 to 99 (39 residues), 59.9 bits, see alignment (E = 9.1e-20) TIGR00487: translation initiation factor IF-2" amino acids 413 to 993 (581 residues), 852.8 bits, see alignment E=1.6e-260 TIGR00231: small GTP-binding protein domain" amino acids 497 to 651 (155 residues), 111.4 bits, see alignment E=3.7e-36 PF00009: GTP_EFTU" amino acids 497 to 653 (157 residues), 135.6 bits, see alignment E=6.2e-43 PF01926: MMR_HSR1" amino acids 498 to 603 (106 residues), 40.8 bits, see alignment E=8.7e-14 PF00071: Ras" amino acids 499 to 654 (156 residues), 28 bits, see alignment E=5.9e-10 PF22042: EF-G_D2" amino acids 669 to 747 (79 residues), 109.5 bits, see alignment E=2.8e-35 PF11987: IF-2" amino acids 769 to 883 (115 residues), 138.6 bits, see alignment E=3.7e-44

Best Hits

Swiss-Prot: 65% identical to IF2_BURM1: Translation initiation factor IF-2 (infB) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 65% identity to bmj:BMULJ_01485)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (993 amino acids)

>GFF222 Translation initiation factor 2 (Variovorax sp. SCN45)
MSSTTVAEFANELKKTPETLLDQLKSAGVPKAAATDALTEADKQRLLGHLKASHGTVEPE
RKKITLTKKSTSEIKQADATGRARTIQVEVRKKRTFIQREDGHPASPEAAPQVAEAPAAA
PAAPRVDEAELARREEEARRQAELIRRQEEELAEKRRLREEAEAREREQAEKAERAEQAE
QEAARVAAEKKAAAEAAAAAAAAKNTPAKPAAPAPAPAVTAAAAAAEQQAADTKLAAQNA
ATQAKEDAKAKAAAESKARADEEAARAKDLDERRRKALAEAEAIRAMMNAPARVLVPHKA
PEKPQPEKAAVKGTLHKPATPAARPGAPAAPGAAAAPGAAAGAGKEVKSAKLSSSWAGDP
AKKKEIKTRGDASGGVGRGNWRGGPRGRRGNDRGGHDEQHAPAAPVEARILEVHVPETIT
VAELAHKMAVKAQEVIKQLMKLGQMATINQSLDQDTAMILVEEMGHNAVVAALDDPEAFT
DEDVSAQTAEALPRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETE
RGMVSFLDTPGHEAFTAMRARGAQATDIVILVVAADDGVMPQTKEAIKHAKAAGVPIVVA
INKVDKPDANLDRVKQELVAEEVVPEEYGGDVPFVPVSAKTGQGIDDLLEQVLLQAEVLE
LKAPVDAAAKGLVIEAQLDKGRGPVATVLVQSGTLKTGDVVLAGSTYGRVRAMLDEDGKA
TKAAGPSIPVEIQGLTEVPQAGDEFMVMSDERRAREIATYRAGKFRNTKLAKAQAANLQN
MFTDLSAGEVQTLRIIIKADVQGSQEALAQSLLKLATDEVKVQVVYAGVGGISESDINLA
IASKAVVIGFNVRADAGARKLAEGNGVQLNYYSIIYDAVDEIKVAMSGMLAPERREEIIG
SAEIRTVFVASKIGTVAGSYITSGSVNRSAHFRLLRDNVVVYTGEVDSIKRMKDDVREVR
EGFECGIKLKNYNDIKEGDQLEFFEIKEIARTL