Protein Info for GFF2212 in Pseudomonas sp. DMC3
Annotation: Toluene efflux pump membrane transporter TtgB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to TTGB_PSEPK: Probable efflux pump membrane transporter TtgB (ttgB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 65% identity to abo:ABO_0964)MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592
Predicted SEED Role
"Multidrug efflux RND transporter MexD" in subsystem Multidrug Resistance Efflux Pumps
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1054 amino acids)
>GFF2212 Toluene efflux pump membrane transporter TtgB (Pseudomonas sp. DMC3) MSKFFIDRPIFAWVIALVIMLVGALSILKLPINQYPSIAPPAIAIQVTYPGASAQTVQDT VVQVIEQQLNGIDNLRYVSSESNSDGSMTITATFEQGTNSDTAQVQVQNKLNLATPLLPQ EVQQQGIRVTKAVKNFLLVIGVVSRDGSMTKDDLSNYIVSNMQDPISRTAGVGDFQVFGA QYAMRIWLDPAKLNNFNLTPVDVKNAIAAQNVQVSSGQLGGLPAVQGQQLNATIIGKTRL QTAEQFKEILLKVNKDGSQVRLKDVAEVGLGGENYAISAQFNGSPASGLAVKLANGANAL DTAKALRNTIDSLKPFFPQGMEVVFPYDTTPVVTESIKGVVHTLVEAIALVFLVMFLFLQ NFRATVITTMTVPVVLLGTFGILAAAGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM SEEGLSPKEATKKSMGQIQGALVGIALVLSAVLLPMAFFSGSTGVIYKQFSITIVSAMAL SVLVALIFTPALCATMLKPIPKGEHGTPKKGFFGWFNRNFDRGVRSYERGVGNMLTRKAP YLLAYLLIVVGMIWLFTRIPTAFLPEEDQGVLFAQVQTPAGSSAQRTQVVVDEMREYLLR PNKDGGEADAVASVFTVTGFNFAGRGQSSGMAFIMLKPWGERNADNSVFNLAARAQQHFF SFRDAMVFAFAPPAVLELGNATGFDVFLQDRAGIGHEKLMEARNQFLGMAAQSKVLTQVR PNGLNDEPQFQLEIDDEKASALGITISDINNTLSIALGSSYVNDFIDRGRVKKVYVQGQP DSRMSPEDLKKWYVRNAEGTMVPFSAFAKGEWVYGSPKLARYNGVEAVEVLGAPAPGYST GEAMAEVEAIAKKLPSGVGISWTGLSYEERLSGSQAPALYALSLLMVFLCLAALYESWSI PIAVMLVVPLGIIGALMATSLRGLSNDVYFQVGLLTTIGLAAKNAILIVEFAKELHEQGR SLREAAIEACRMRLRPIIMTSLAFVLGVVPLAISTGAGSGSQHAIGTGVIGGMLTATILA IFWVPLFFVTVSSIGQRKKTDADETTETPKEAGQ