Protein Info for GFF2211 in Pseudomonas sp. DMC3

Annotation: putative efflux pump outer membrane protein TtgC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 9 to 465 (457 residues), 489.3 bits, see alignment E=6e-151 PF02321: OEP" amino acids 65 to 255 (191 residues), 85.3 bits, see alignment E=2.4e-28 amino acids 283 to 463 (181 residues), 106.4 bits, see alignment E=8.3e-35

Best Hits

Swiss-Prot: 77% identical to TTGC_PSEPK: Probable efflux pump outer membrane protein TtgC (ttgC) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 98% identity to pfo:Pfl01_1278)

Predicted SEED Role

"RND efflux system, outer membrane lipoprotein, NodT family" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (486 amino acids)

>GFF2211 putative efflux pump outer membrane protein TtgC (Pseudomonas sp. DMC3)
MSKSLLSIAVAAFVLSGCSLIPDYQQPEAPVAGQYPQGPAYSPAQAPAQAAAEQGWKQFF
HDPALQQLIQVALENNRDLRVAALNIDAYAAQYRIQRADLFPAVSANAAGSRQRLPARAS
QTGESMISSSYSATVGVSAYELDLFGRVRSLSEQALQQYFATEEARRSTQISLVANVANA
YLTWQADKELLKLTQDTLGAFEESYKLTSRSNEVGVASALDLAQSRTSVENARAQLARYT
RQVAQDENSLTLLLGTGIPANLQTAKPLSDDLLSDVPAGLPSDLLQRRPDILQAEYNLKA
ANANIGAARAAFFPSISLTANAGSLSPDLSGLFKGGSGTWLFQPQINLPIFNAGSLRASL
DYAKIQKDIGVANYEKSIQTAFQEVADGLAARQTYTEQLQAQRDFVQANQDYYRLAERRY
RIGVDSNLTFLDAQRQLFSAQQALITDRLAQLTSAVNLYKALGGGWNAQTAQNEALKEEA
PKMKLF