Protein Info for GFF2191 in Methylophilus sp. DMC18

Annotation: Phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 24 to 101 (78 residues), 116 bits, E=5.5e-38
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 140 to 591 (452 residues), 717.4 bits, E=2.6e-220
• PF01964: ThiC_Rad_SAM
  • amino acids 142 to 587 (446 residues), 619 bits, E=4e-190

Best Hits

Swiss-Prot: 81% identical to THIC_NITMU: Phosphomethylpyrimidine synthase (thiC) from Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 94% identity to mmb:Mmol_0035)

MetaCyc: 74% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (626 amino acids)

>GFF2191 Phosphomethylpyrimidine synthase (Methylophilus sp. DMC18)
MNAIDKNIKFVNADAKVDEAATKPFAKSRKIYVEGSRPDIRVPFREISLSDTPSSFGGDT
NPPVVVYDTSGPYTDPNVEIDIRNGLPALRAQWIMERGDVEQLDGPTSEFGHQRLVDPEL
SQMRFNLHRKPLRAKAGKNVSQMHYARQGIITPEMEFIAIRENQRRENMSELLQTQHPGQ
NYGASIPKVITPEFVRDEVARGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSALGSS
ISEEVEKMVWGTRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAE
DLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYIPMTAKRMTGIVSRGGSIMAKWCLAHHK
ESFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIYDANDEAQFAELKTLGELTQIAWKHDV
QCMIEGPGHVPMHLIKENMDLQLEHCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWY
GCAMLCYVTPKEHLGLPDKEDVRVGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFR
WEDQFNLGLDPEKAKEFHDETLPQEGAKQAHFCSMCGPHFCSMKITQDVRDYADKLGVDE
QVALEKGMQEKAIEFVKKGAEVYQKV