Protein Info for GFF2188 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: RND efflux system, inner membrane transporter CmeB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to ACRD_ECOLI: Probable aminoglycoside efflux pump (acrD) from Escherichia coli (strain K12)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 64% identity to abo:ABO_0964)MetaCyc: 63% identical to multidrug efflux pump RND permease AcrF (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-367
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1037 amino acids)
>GFF2188 RND efflux system, inner membrane transporter CmeB (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MANFFIDRPIFAWVLAILLCLTGALAIFSLPVEQYPDLAPPNVRITANYPGASAQTLENT VTQVIEQNMTGLDNLMYMSSQSSGTGQATITLSFIAGTDPDEAVQQVQNQLQSAMRKLPQ AVQDQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVASNIQDPLSRVNGVGDIDAYGS QYSMRIWLDPAKLNSFQMTTKDVTDAIESQNAQIAVGQLGGTPSVDKQALNATINAQSLL QTPQQFRDITLRVNQDGSEVKLGDVATVELGAEKYDYLSRFNGNPASGLGVKLASGANEM ATAKLVLDRLNELAQYFPHGLEYKIAYETTSFVKASIIDVVKTLLEAIALVFLVMYLFLQ NFRATLIPTIAVPVVLMGTFSVLYAFGYSINTLTMFAMVLAIGLLVDDAIVVVENVERIM SEEGLTPREATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGTTGAIYRQFSITIVSAMVL SVLVAMILTPALCATLLKPLHKGEQHGQRGFFGWFNRTFNRNAERYEKGVAKILHRSLRW ILIYVLLLGGMVFLFLRLPTSFLPQEDRGMFTTSIQLPSGSTQQQTLKVVEKVENYYFTH EKDNIMSVFSTVGSGPGGNGQNVARMFVRLKDWDARDPTTGSSFAIIERATKAFNQIKEA RVFASSPPAISGLGSSAGFDMELQDHAGAGHDALMAARDQLIELAGKNSSLTRVRHNGLD DSPQLQIDIDQRKAQALGVSIDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAKYRML PDDINLWYVRNKDGGMVPFSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMD VMESLVHQLPGGFGLEWTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVM LVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHALLDA TLYASRQRLRPILMTSLAFIFGVLPMATSTGAGSGSQHAVGTGVMGGMISATVLAIFFVP LFFVLIRRRFPLKPRPK