Protein Info for GFF2175 in Methylophilus sp. DMC18

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 PF08886: GshA" amino acids 17 to 428 (412 residues), 640.6 bits, see alignment E=3.9e-197 TIGR02049: glutamate--cysteine ligase" amino acids 18 to 429 (412 residues), 620.4 bits, see alignment E=5.9e-191

Best Hits

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 77% identity to mmb:Mmol_2263)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2), divergent, of Alpha- and Beta-proteobacteria type" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (441 amino acids)

>GFF2175 hypothetical protein (Methylophilus sp. DMC18)
MVPHLTTALSGPLYTLEKRVLQAMPDIEHWFRTKWQEIPAPFYASVDLRNAGFKIAPVDT
NLFPGGFNNLNPEFLSLAVQAAQMAVEKICPDARRLLLIPENHTRNTYYLRNVVELCHIL
KTAGMEVRVGSLSPEITEITKLETHDGLPLVLEPLVRVNNRLQLKAATFEGVEYPAFDSC
AILLNNDLSGGVPDLLQGLEQTLIPPLHAGWFSRRKSQHFSAYNTVVDEFSQLLQIDPWL
LNPYFDTASGLDFHARVGEDLLADKVEAILKKTQAKYDEYGITQPPFAIVKADAGTYGMG
IMSVKSADEVRDLNRKARNKMSVIKEGMQVSEVMIQEGVYTFESINDAVAEPVVYMMDHF
VIGGFYRVHTGRGVDENLNAPGMHFAPLAFEKPCTLPDCAGAPDAPPNRFYAYGVVARLA
LLAAGLELQARDLPENDLLTA