Protein Info for GFF2170 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: N-methylhydantoinase A (EC 3.5.2.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF05378: Hydant_A_N" amino acids 14 to 188 (175 residues), 166.3 bits, see alignment E=8.8e-53 PF01968: Hydantoinase_A" amino acids 209 to 500 (292 residues), 313.6 bits, see alignment E=1.9e-97 PF19278: Hydant_A_C" amino acids 515 to 685 (171 residues), 76.5 bits, see alignment E=2.8e-25

Best Hits

KEGG orthology group: K01473, N-methylhydantoinase A [EC: 3.5.2.14] (inferred from 89% identity to vap:Vapar_5966)

Predicted SEED Role

"N-methylhydantoinase A (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14

Use Curated BLAST to search for 3.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (698 amino acids)

>GFF2170 N-methylhydantoinase A (EC 3.5.2.14) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSNTNLTPHDARLRVAVDIGGTFTDMAAFDEVTGQLLFGKALSTHGQLVNGIQATLDSAD
IDLRDGHLFLHGSTIAINTLLERNGAKTALLITEGFRDIYEIGRVNRPDAYNLFFNKHEP
LVKRSLRYEVAERLRADGSTHKALDEAAVRELARELGGKNIEAVAVLLLHSYRNPAHEQR
VKQILQEELPGAFVCASHELSQEYREFERVSTAVANAYVGPRVSAYLGQLEEHLSHKGFE
GDFYVVQSTGGLFPSEHARRDCVRMLESGPAAGVIGAQAICAQLGMGDAIAFDMGGTTAK
AGVISEGRPLTTGSALIGGYERALPIQIPMMDIHEVGTGGGSIARVETGNALRVGPQSAG
SIPGPVAYGRGGTEPTVTDANLVLGRLDADHFLGGELKLDLAGTQRQMEERVAKPLGLDA
TAAADGILRIAVTQMSHAVKAVTTERGLDAGSFTMVVYGGAGPLHASAIAREIGIRKVLI
PYAPGYFSAYGMLFSDLRYDYVRSVFRKLNDVSFDEIEALYKGMEDEGRAALAASGITPD
GIVIERAADMRYVGQEHAVTVDLPGAFFVDKDRSAIKNHFDQVHKVRYGTSAPQEAADLV
SLRVTVLGTMKKPPRHHVDEGAALPEAAALRGTKPVYFRAGGWADTPVYQRDALRAGNVI
AGPALIEEHASTTVVQPGDELRVDGLGNLQIAIGSDRS