Protein Info for GFF2165 in Variovorax sp. SCN45

Annotation: Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00890: FAD_binding_2
  • amino acids 9 to 547 (539 residues), 330.9 bits, E=3.6e-102
• PF01266: DAO
  • amino acids 9 to 56 (48 residues), 45 bits, 2.8e-15
• PF12831: FAD_oxidored
  • amino acids 9 to 66 (58 residues), 38.4 bits, 2.4e-13
• PF13450: NAD_binding_8
  • amino acids 12 to 47 (36 residues), 33.4 bits, 1.1e-11

Best Hits

KEGG orthology group: K05898, 3-oxosteroid 1-dehydrogenase [EC: 1.3.99.4] (inferred from 70% identity to bbt:BBta_4671)

Predicted SEED Role

"Succinate dehydrogenase/fumarate reductase, flavoprotein subunit"

MetaCyc Pathways

• testosterone degradation (anaerobic) (1/6 steps found)
• androstenedione degradation I (aerobic) (7/25 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• androstenedione degradation II (anaerobic) (5/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (10/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (6/49 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.99.4

Protein Sequence (586 amino acids)

>GFF2165 Succinate dehydrogenase/fumarate reductase, flavoprotein subunit (Variovorax sp. SCN45)
MEQNTFECDVLVVGSGASGMSTAITAASQGLKVLVVDKEPRFGGTTARSGGWLWIPGTRL
ATEQGIHEPEGAALAYMKHETTTHFDAARVGAFLDMGPKAIDFFTRNTCVQFDMPAVFPD
YHAEAPGGQQGGRSMVTRPFDGRELGKRVKQLAPPLPELTVFGMMLGSGPEIKHFMRAFK
SPTSFWYVTKRLTRHFFDVLGHGRGMTLTNGNALAGRLAKAAMDLDIPVWLSSPVKKLVV
EYDGVSGAVVQHEGKTVRVVAKRGVVLACGGFPYDVERRKQLFPHAPTGKEHYSPSPSTN
TGDGLRLAEAVGGRVDGTIPHAAAWVPASVTTRPDGTKGVMPHFIDRAKPGVIAVTPKGK
RFANEGNSYHDFVQAMVKACEGEPEVTAWLLCDHKALRNYGLGCVAPSPLPIGRHLKSGY
LKRGATVGELARQIGIAPAVLEATVQEFNKGASTGDDPAFGKGSKAYNRYQGDANVTPNP
CVAPLEHGPYYAIKLVIGDIGTFAGLVTDEKTRVLDANRQPIKGLYAVGNDAASVMGGNY
PGAGITLGPALTFGYVAGLQLAQAEAAAAVQLKVEQREAVPVSRKA