Protein Info for GFF2165 in Pseudomonas sp. DMC3

Annotation: Pyridoxine/pyridoxamine 5'-phosphate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00558: pyridoxamine 5'-phosphate oxidase
  • amino acids 3 to 215 (213 residues), 294.5 bits, E=2.3e-92
• PF12766: Pyridox_oxase_2
  • amino acids 30 to 116 (87 residues), 40.3 bits, E=6.2e-14
• PF01243: PNPOx_N
  • amino acids 46 to 154 (109 residues), 102.8 bits, E=2.5e-33
• PF10590: PNP_phzG_C
  • amino acids 175 to 215 (41 residues), 70.3 bits, 1.5e-23

Best Hits

Swiss-Prot: 94% identical to PDXH_PSEPF: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 94% identity to pfo:Pfl01_1229)

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• pyridoxal 5'-phosphate salvage I (5/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (5/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.5

Protein Sequence (215 amino acids)

>GFF2165 Pyridoxine/pyridoxamine 5'-phosphate oxidase (Pseudomonas sp. DMC3)
MTQALADMRRDYTRDGLTEAQAPTEPFALFHQWFADAVKTEQAPVEANAMTLATVDADGR
PHCRILLLKGLDEQGFTFFTNYDSAKGQHLAANPFAAMTFFWPTLERQVRIEGRVVKVTA
QESDAYYQVRPLGSRLGAWASPQSRVINGRGELEDLLKATEQRFSDSQPHCPEHWGGYRL
LPERIEFWQGRPSRLHDRLNYRLQGTEWILERLAP