Protein Info for GFF2161 in Pseudomonas sp. DMC3

Annotation: ATP-dependent DNA helicase DinG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 transmembrane" amino acids 472 to 492 (21 residues), see Phobius details PF00270: DEAD" amino acids 58 to 117 (60 residues), 24.6 bits, see alignment 1.9e-09 PF13307: Helicase_C_2" amino acids 553 to 708 (156 residues), 144 bits, see alignment E=5e-46

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 98% identity to pfo:Pfl01_1225)

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (714 amino acids)

>GFF2161 ATP-dependent DNA helicase DinG (Pseudomonas sp. DMC3)
MISTELKTTIQGAYSRFLEAKSLKPRYGQRLMIAEIAKVLGDIDTDDEGRRSGDPAIVAV
EAGTGTGKTVAYSLAAIPTAKLAGKRLVIATATVALQEQIVYKDLPDLMRNSGLNFSFAL
AKGRGRYMCLSKLDMLLQEGHAQTATAQLFEEEGFKIEVDEASQKLFTSMIEKLAGNKWD
GDRDSWPNALEDADWARLTTDHSQCTNRHCPNFGQCAFYKAREGMGKVDVIVTNHDMVLA
DLALGGGAVLPDPRDTIYVFDEGHHLPDKAIGHFAHYTRLRSTADWLETTAKNLTKLLAQ
HPLPGDLGKLIEQVPELAREIKTQQQFMFTACEQIADFKPGEDVEGRERPRHRFVGGVIP
EDMREMGIELKKGYSRLNDLFQRLTDLLKEGMDGEVNIGIASNQAEEWYPLFGSLLSRAS
GNWELWTAFTAEDPEDSPPMARWLTLAESGSLFDIEVNASPILAAETLRRSLWNVAYGCL
VTSATLTALGTFDRFRMRAGLPKKAVTAVVPSPFHHADAGVLRVPDLKADPRDAAAHTAA
IIRELPDLVEGSRGTLVLFSSRKQMQDVFDGLDRDWRKQVFIQGNLSKQETLNKHKARVD
GGDSSVLFGLASFAEGVDLPGAYCEHVVIAKIPFSVPDDPVEAALSEWIEARGGNPFMEI
SVPDASLKLVQACGRLLRTEEDRGTITLLDRRLVTQRYGKAILNALPPFRREIS