Protein Info for GFF2154 in Variovorax sp. SCN45

Annotation: twitching motility protein PilJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 768 transmembrane" amino acids 99 to 120 (22 residues), see Phobius details amino acids 383 to 408 (26 residues), see Phobius details PF13675: PilJ" amino acids 122 to 225 (104 residues), 68.8 bits, see alignment E=4.2e-23 amino acids 256 to 355 (100 residues), 38.6 bits, see alignment E=9.9e-14 PF00015: MCPsignal" amino acids 550 to 733 (184 residues), 136.8 bits, see alignment E=7.2e-44

Best Hits

KEGG orthology group: K02660, twitching motility protein PilJ (inferred from 93% identity to vpe:Varpa_5224)

Predicted SEED Role

"twitching motility protein PilJ"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (768 amino acids)

>GFF2154 twitching motility protein PilJ (Variovorax sp. SCN45)
VSSIADKFKKLLPANSGNDKAPVDGSGAISLDDVDTVQVPDEKTVKLARKSGAADTSFGD
VDLPGGGDAANEPGAAALDAVPDTAGGRVGAAPAKQQRILAIMLAVVVLLLLLVAGSAIL
RAERLAQQVAATGQALMQSQRLAKSISQALVGSASAFVEVKDSADDLSRRVQGLTNGDES
LRLERVGNQYDEEMNKINPLVTRADTSAKAVLAQRQILTQVGAALRDINQQSSTLLEMTE
TVASLKMQQNATLPEISAAGQLVMLTQRIGKSTNEFFTVEGVNPDAVFLLGKDLNTFQET
TRGLLDGNAQQRFPGSKDPQTRQQLEAILKTYEQMRTQASAILGNLQGLVAAREAQASIL
TDSEPLRKSLGDLQDKLSARTGLGAGTIVLLFVLSLAALALAGAIGFVQVREGRERAAVA
ERERLAAEQASEEAGRVNNANQAAILRLMNELQTVAEGDLTQEATVTEDITGAIADSVNY
TVEELRLLVGNVQNTATRVAQTTSQVESTSTELLAASTEQLREIRETGQSVLTMAERING
VSSQAQESATVARQSLQAASSGLQAVQNAIGGMNSIRDQIQETSKRIKRLGESSQEIGEI
TELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSADATRQISALVKAI
QTDTQDAVGAMERSTQGVVEGAKLSDNAGTALSEIDRVSRRLADLIEQISQSASREADSA
NVVAANIQHIFAVTEQTGEGTRTTAQQVRELSQMAEELRRSVARFKIA