Protein Info for GFF2148 in Sphingobium sp. HT1-2

Annotation: Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 PF12573: OxoDH_E1alpha_N" amino acids 19 to 59 (41 residues), 55.3 bits, see alignment 4.5e-19 PF00676: E1_dh" amino acids 96 to 391 (296 residues), 329.4 bits, see alignment E=1.7e-102

Best Hits

Swiss-Prot: 60% identical to ODBA_PSEAE: 2-oxoisovalerate dehydrogenase subunit alpha (bkdA1) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00166, 2-oxoisovalerate dehydrogenase E1 component, alpha subunit [EC: 1.2.4.4] (inferred from 87% identity to sch:Sphch_1205)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>GFF2148 Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) (Sphingobium sp. HT1-2)
MTDSIESGAVTDGQGRNLPPLQLHVPEPPARPGESADFTHLDIPAADAAPRPDEATHPSQ
MRDFAYGLVRVLDEDGHAVGAWDPKLPADTLLRMLRYMALTRAFDGRMFRAQRQGKTSFY
MKSTGEEAVSIGAALALSRDDMCFPSYRQQGILIARDWSIVDMMNQIYSNKGDRMKGRQL
PIMYSAREAGFFSISGNLTTQYPQAVGWAMASAAKGDTRIAATWCGEGSTAEGDFHSACT
FASVYRAPVIMNVVNNQWAISSFSGFAGAEATTFAARAIGYGIAGLRVDGNDVLAVYAAT
RWAADRARANGGPTLIEHFTYRVEGHSTSDDPTAYRSAEESSKWPLGDPIARLKQHCITL
GIWDEERHAAMDQELAEMVRDAAREAEKNGILGHGLHHPMESMFQDVFEDMPWHLKEQQQ
QMLDEWKAAGL