Protein Info for GFF2141 in Variovorax sp. SCN45

Annotation: GTP-binding and nucleic acid-binding protein YchF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00092: GTP-binding protein YchF
  • amino acids 1 to 362 (362 residues), 508 bits, E=8.2e-157
• PF02421: FeoB_N
  • amino acids 5 to 46 (42 residues), 31 bits, 2.7e-11
• PF01926: MMR_HSR1
  • amino acids 5 to 109 (105 residues), 73.2 bits, E=2.9e-24
• PF06071: YchF-GTPase_C
  • amino acids 279 to 361 (83 residues), 119.1 bits, E=1.1e-38

Best Hits

Swiss-Prot: 67% identical to YCHF_ECOLI: Ribosome-binding ATPase YchF (ychF) from Escherichia coli (strain K12)

KEGG orthology group: K06942, (no description) (inferred from 98% identity to vap:Vapar_4635)

MetaCyc: 67% identical to redox-responsive ATPase YchF (Escherichia coli K-12 substr. MG1655)
Nucleoside-triphosphatase. [EC: 3.6.1.15, 3.6.1.5]

Predicted SEED Role

"GTP-binding and nucleic acid-binding protein YchF" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5

Protein Sequence (363 amino acids)

>GFF2141 GTP-binding and nucleic acid-binding protein YchF (Variovorax sp. SCN45)
MSLQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDPRLDQLAEIVKP
ERVVPAIVEFVDIAGLVAGASTGEGLGNKFLAHIRETDATVNVVRCFDDENVIHVAGKVD
PISDIEVIQTELCLADLATVEKALHRHTKVARSGDKDAQKLVGLLERCQAALNENTPVRA
LEFTKEEQPLVKSFTLITAKPAMFVGNVSEDGFENNPYLDRLREYAAKQGAPVVAICAKI
EADLAEMDDEDKKMFLAEIGQEEPGLNRLIRAAFKLLGLQTYFTAGVKEVRAWTIHIGDT
GPQAAGVIHGDFEKGYIRAQTIAFEDYIAFKGEQGAKDAGKMRSEGKEYVVKDGDVMNFL
FSS