Protein Info for GFF2140 in Xanthobacter sp. DMC5

Annotation: Inner membrane transport protein RhmT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 signal peptide" amino acids 1 to 41 (41 residues), see Phobius details transmembrane" amino acids 50 to 69 (20 residues), see Phobius details amino acids 84 to 103 (20 residues), see Phobius details amino acids 110 to 134 (25 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 177 to 199 (23 residues), see Phobius details amino acids 245 to 266 (22 residues), see Phobius details amino acids 278 to 300 (23 residues), see Phobius details amino acids 312 to 330 (19 residues), see Phobius details amino acids 336 to 356 (21 residues), see Phobius details amino acids 363 to 385 (23 residues), see Phobius details amino acids 401 to 421 (21 residues), see Phobius details PF07690: MFS_1" amino acids 23 to 387 (365 residues), 161.2 bits, see alignment E=1.7e-51

Best Hits

Swiss-Prot: 52% identical to RHMT_ECOLI: Inner membrane transport protein RhmT (rhmT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 71% identity to mno:Mnod_6622)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>GFF2140 Inner membrane transport protein RhmT (Xanthobacter sp. DMC5)
MPAPSASLQATISKVTKRLLPFLLLMYVLAFLDRANVGFAKKAFQADTGISDAAFALGAS
IFFLSYALLEVPSNIIMHKVGARIWMARIMVTWGLISAAMMFAHNEFTFYVLRVLLGAAE
AGFFPGVIFYLTLWFPSFARGRAMGLFYFGAPLAFIFGSPLSGLLLDWHGLGGLHGWQWM
FMVEGLLATVVGVWAYFYLDNKPADAKWLSAAEKDELTRVLAAEESHKTTHGPAHLLSAM
RNMKVLHFALIYFLIQMSVYGVVFYLPTQVANLLGTKVGLQVGFVSAIPWVCALVAAYVL
PRMADASGNRRGYAAAILAVSGLGIAASVATTSPALALIALCFAAAGFISVQPIFWTFPT
DYLGGVAAAGGIALINSLGALGGFVAPNVKTWAEAAFASQAAGLYVLAGTTLLGALLILA
IGQSRALPVAPATRRA