Protein Info for GFF2134 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 29 to 30 (2 residues), see Phobius details amino acids 53 to 69 (17 residues), see Phobius details amino acids 90 to 107 (18 residues), see Phobius details amino acids 113 to 135 (23 residues), see Phobius details amino acids 154 to 174 (21 residues), see Phobius details amino acids 188 to 210 (23 residues), see Phobius details amino acids 231 to 252 (22 residues), see Phobius details amino acids 276 to 295 (20 residues), see Phobius details amino acids 317 to 342 (26 residues), see Phobius details amino acids 357 to 376 (20 residues), see Phobius details amino acids 383 to 402 (20 residues), see Phobius details amino acids 408 to 434 (27 residues), see Phobius details amino acids 446 to 465 (20 residues), see Phobius details amino acids 473 to 496 (24 residues), see Phobius details PF13000: Acatn" amino acids 20 to 149 (130 residues), 28.8 bits, see alignment E=4.9e-11 PF07690: MFS_1" amino acids 21 to 321 (301 residues), 42.8 bits, see alignment E=3.5e-15 amino acids 324 to 513 (190 residues), 44.3 bits, see alignment E=1.2e-15 TIGR00901: AmpG-like permease" amino acids 29 to 455 (427 residues), 430.1 bits, see alignment E=3.8e-133

Best Hits

KEGG orthology group: K08218, MFS transporter, PAT family, beta-lactamase induction signal transducer AmpG (inferred from 95% identity to pfo:Pfl01_1198)

Predicted SEED Role

"AmpG permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>GFF2134 hypothetical protein (Pseudomonas sp. DMC3)
MPRKTWRAALAAYASPSTLVLLLLGFAAGLPYMLVFSTLSVWLREAGVARETIGYASLIG
LAYAFKWVWSPLLDQWRLPLLGKLGRRRSWLVLSQALVILGLIGMGFCDPQKHLSWLIAI
AVVVAFASATQDIAVDAYRLEIADDSRQAALAASYMSGYRVAALLATAGALFFAEGFGST
GFNYKHAAWTGTYVLFGALMVPALLTTLFMREPPVPLRTQLQAGRYTFMHQLMSVFVLIV
LLVSVPAMFTQLYNTDFAGVLFGDMSPLDLLLEDRAFLRAILYILLTGMCLSAMGRRGLA
PVLTPVNDFILRYRWQALLLLGLIATYRMSDTVMGVMANVFYIDQGFTKDQIASVSKIFG
LIMTLVGAGMGGLLIVRFGILPILFIGGVSSAATNLLFVMLADMGPNLQMLVVTISLDNF
SSGLATSAFVAYLSSLTNLKFSATQYALLSSIMLLLPRLMGGYSGVLVEKFGYHSFFLIT
ALLGVPTLLLIALHWFQERRREGQTPTPEPAPTPVTEES