Protein Info for GFF213 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Guanine deaminase (EC 3.5.4.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02967: guanine deaminase
  • amino acids 21 to 425 (405 residues), 478.5 bits, E=8.4e-148
• PF01979: Amidohydro_1
  • amino acids 63 to 426 (364 residues), 164 bits, E=6.1e-52
• PF07969: Amidohydro_3
  • amino acids 115 to 426 (312 residues), 43.4 bits, E=3.4e-15

Best Hits

Swiss-Prot: 39% identical to GUAD_DEIRA: Probable guanine deaminase (guaD) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)

KEGG orthology group: K01487, guanine deaminase [EC: 3.5.4.3] (inferred from 75% identity to adn:Alide_1067)

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3)" in subsystem Purine Utilization or Purine conversions (EC 3.5.4.3)

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• superpathway of purines degradation in plants (12/18 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3

Protein Sequence (426 amino acids)

>GFF213 Guanine deaminase (EC 3.5.4.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNAYRAALLRFDDQQRPVYDADGLLVIGPDAQGRRVVRAAGDHAALIGNYAGVSVEDLSG
QLIAPGFIDMHVHYPQTDVIGAPAAGLLPWLENYTFPHESRFHEPDHAREVAAFFLDELS
RNGVTTALTFATSHPASVDAMFEAARSRGLRFVTGKVLQDRHSPDGVRDQTEQSLIDTEA
LIQRWHGVDRLGYAITPRFAPTSTPEQLRGAGELAAKYGDVWVQSHVAENRDEVRWAAEL
FPKSRSYLGVYGDFGLLRQRAVYAHCIHLDQADRELMAATGTTAAVSPTSNQFLGSGFFD
YLAADAAGMGYGLASDVGGGTSFSPFRTMLAAYCVGREGQTKPGLSLTPGQLWWQHTAGA
ARALGLEGVVGNLQPGHEADFVVLDPQATPLLARKTSQANSLDELLFALIVLGDDRVVAR
TVVAGR