Protein Info for PS417_10820 in Pseudomonas simiae WCS417

Annotation: N-methylproline demethylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF00724: Oxidored_FMN" amino acids 6 to 340 (335 residues), 170.3 bits, see alignment E=2.2e-53 PF07992: Pyr_redox_2" amino acids 387 to 641 (255 residues), 37.8 bits, see alignment E=4.6e-13 PF03486: HI0933_like" amino acids 387 to 427 (41 residues), 27.2 bits, see alignment 5.1e-10 PF00070: Pyr_redox" amino acids 387 to 421 (35 residues), 24.2 bits, see alignment (E = 1.2e-08) PF13450: NAD_binding_8" amino acids 390 to 425 (36 residues), 30.5 bits, see alignment 1.1e-10

Best Hits

Swiss-Prot: 77% identical to STCD_RHIME: Probable N-methylproline demethylase (stcD) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 91% identity to pba:PSEBR_a3629)

MetaCyc: 77% identical to N-methyl-L-proline N-demethylase (Paracoccus denitrificans PD1222)
1.5.3.-

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TYF0 at UniProt or InterPro

Protein Sequence (678 amino acids)

>PS417_10820 N-methylproline demethylase (Pseudomonas simiae WCS417)
MSSDPLLQPYKIKHLTLRNRIMTTAHEPAYPVDGMPQALYRAYHVERAKAGVALTMTAGS
AAVSRDSPPVFNNVLAYKDEVVGWLKDLTDECHEHGAAVMIQLTHLGRRTRWDKADWLPV
VSPSHRREASHRSFPKKLEDWDIERIIKDYVDAAERMKAAGLDGLELQAYGHLMDQFWSP
LTNELEGPYGGSLENRMRFTFDVLRGIRQRVGEDFLLGVRYTGDEALPGGFTANEGLQVS
HMLKDSGLVDFLNVVRGHIDTDAGLTDVIPIQGMRNSPHLEFAGEIRASTGFPTFHAAKI
PDVATARYAIASGKVDMVGMTRAHMTDPHIVRKIIERREEDIRPCVGANYCLDRIYQGGA
AYCIHNAATGRETSMPHDIPKAAVKRKVLVIGTGPAGLEAARVAGERGHEVTVLEAADRP
GGQIRLTALSERRREMIGIIDWRMAQCERLGVTFHFNTWAEAETVLAHEPDVVIVATGGM
PHTEVLAEGNELVVSTWDIISGDVKPGRHVLIFDDAGDHAALQAAEVIAQSGASVEIVTP
DRAFAPEVMAMNLVPYMRSLQDLDVIFTVTYRVESVEKRDGRLVARFGSDYGTVHKQRVV
DQVVINHGTVPLDDLYFDLRAHSSNGGAVEQHDLIAGNVQNIVTHAEGRFQLFRIGDAVA
ARNTHAAIYDALRLVKDL