Protein Info for GFF2114 in Variovorax sp. SCN45

Annotation: 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 PF00501: AMP-binding" amino acids 23 to 403 (381 residues), 256.9 bits, see alignment E=2.9e-80 PF13193: AMP-binding_C" amino acids 453 to 527 (75 residues), 66.3 bits, see alignment E=3.8e-22

Best Hits

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 92% identity to vpe:Varpa_5261)

Predicted SEED Role

"Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-)" (EC 6.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (546 amino acids)

>GFF2114 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type (Variovorax sp. SCN45)
MFGLMQDRPLLISSLIDYAATFHPHAEVVGRTVEGPVHRTNYSEIQRRSKQVANALRTLG
VESGDRVGTLAWNTYRHLALYFGVSGSGAVLHTVNPRLFPEQIDYIVNHAEDKVLFFDTT
FAPLVEKLAPGLKSVRAFIAMTDRAHMPAIDVPNLLCYDELVGAQSEAYDWPEFDERTAS
SLCYTSGTTGNPKGVLYSHRSTVLHSLMECAPDTFGVNSQMTLLLAVPMFHANAWGMPYA
AAMTGMKLVMPGPHMDGQSLYELMRDEKVGFSQAVPTIWLMLFQYFDAHPEIDTRAIGLK
RIGVGGAATPRSMIERFERDFGADFVQGWGMTETSPIGVIGNLLPKHLSMPKEEQLRVKM
RQGRGVWGVELKIVGEDGQRLPHDGKAFGHLHVRGPWIASGYFKSEGGPVLDAEGFFPTG
DVANIDEDGYVQLVDRAKDVIKSGGEWISSIDLENTASLHPAVAEAAVIGIAHPKWQERP
LLIVVKRAGSEVTREELLTFLAERVVKWWLPEDVVFVDSLPHTATGKLLKTKLREQFKGY
TATTSV