Protein Info for GFF2107 in Sphingobium sp. HT1-2

Annotation: NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01972: proton-translocating NADH-quinone oxidoreductase, chain M
  • amino acids 7 to 479 (473 residues), 601.4 bits, E=6.1e-185
• PF01059: Oxidored_q5_N
  • amino acids 69 to 123 (55 residues), 24.8 bits, 2e-09
• PF00361: Proton_antipo_M
  • amino acids 128 to 413 (286 residues), 291.6 bits, E=6.5e-91

Best Hits

Swiss-Prot: 59% identical to NUOM_RHOCA: NADH-quinone oxidoreductase subunit M (nuoM) from Rhodobacter capsulatus

KEGG orthology group: K00342, NADH dehydrogenase I subunit M [EC: 1.6.5.3] (inferred from 92% identity to sjp:SJA_C1-13350)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (517 amino acids)

>GFF2107 NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3) (Sphingobium sp. HT1-2)
MDGFPILSLMMAVPMAGAIACLFAGANNARWIALIATLVDLVLGIVLWVNFDQSGAGAQW
QFTEYAPIFGRFAWALGIDGIALLLIALTVFLMPICIGASWNAINKRVGEYMAAFLFMEV
LMIGVFTAQDLYLFYIMFEAGLIPMYLIIGIWGGANRIYASYKFFLYTLLGSVLMLIAMM
WMVHEAGTTRIPDLMAYNFDPKIQIWLFLAFFASFAVKMPMWPVHTWLPDAHVQAPTAGS
VILAGVLLKMGGYGFIRFSLPMFPEASAQLAPWVWGLSMVAVVYTSLIALVQSDMKKLIA
YSSVAHMAIVTIGLFAFNQAGLEGAMMVMLGHGLVSGALFLCVGVIYDRLHTREINRYGG
LAINMPRYAVLFLLFTMASVGLPGTSNFVGEFLSLMGIYQVSTWAALICTTGIILGAAYM
LYLYRRICYGDQVNADAAAMPDLSAREIWLLAPIAAAVLWMGVYPESFLAPMRPDIRALE
ARLAPAAPAGDSQIKMGAPKPVGEGHHEEAAAHGEAH