Protein Info for GFF2090 in Variovorax sp. SCN45

Annotation: Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 PF05496: RuvB_N" amino acids 32 to 190 (159 residues), 274.4 bits, see alignment E=7.7e-86 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 36 to 338 (303 residues), 491 bits, see alignment E=5.9e-152 PF07728: AAA_5" amino acids 66 to 184 (119 residues), 31.6 bits, see alignment E=4.7e-11 PF00004: AAA" amino acids 67 to 189 (123 residues), 73.2 bits, see alignment E=8.4e-24 PF17864: AAA_lid_4" amino acids 193 to 266 (74 residues), 109.9 bits, see alignment E=1.2e-35 PF05491: RuvB_C" amino acids 268 to 337 (70 residues), 82.1 bits, see alignment E=6.9e-27

Best Hits

Swiss-Prot: 97% identical to RUVB_VARPS: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 97% identity to vpe:Varpa_5284)

MetaCyc: 72% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (354 amino acids)

>GFF2090 Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12) (Variovorax sp. SCN45)
MTIQTDDFAPVPQRVVSAAPASPNEEAIERALRPKLLDEYVGQAKVREQLEIFIGAARKR
KEALDHVLLFGPPGLGKTTLSHIIAAELGVNLRQTSGPVLEKPKDLAALLTNLEPNDVLF
IDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLQPFTLVGATTRAGMLTNPL
RDRFGIVARLEFYTPEELALIVRRSAGLLKVETDAAGGFEIARRSRGTPRIANRLLRRVR
DYAEVKGNGRITEDIAHKALAMLDVDPQGFDLMDRKLLEAVIHRFDGGPVGLDNVAASIG
EERDTIEDVIEPYLIQQGYLQRTPRGRIATLAAYRHLGVAPPSGRGDVPDLFGA