Protein Info for GFF2089 in Xanthobacter sp. DMC5

Annotation: tRNA N6-adenosine threonylcarbamoyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 356 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 6 to 326 (321 residues), 397.4 bits, see alignment E=4.2e-123 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 7 to 319 (313 residues), 318.4 bits, see alignment E=4.9e-99 PF00814: TsaD" amino acids 31 to 320 (290 residues), 307.6 bits, see alignment E=4.6e-96

Best Hits

Swiss-Prot: 68% identical to TSAD_RHOPS: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Rhodopseudomonas palustris (strain BisB5)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 94% identity to xau:Xaut_1157)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (356 amino acids)

>GFF2089 tRNA N6-adenosine threonylcarbamoyltransferase (Xanthobacter sp. DMC5)
LPDLMVLGIETTCDETSAAVVTRSPSGASDIRSNVIRSQVEIHSPYGGVVPEIAARAHVE
VLDHVIAEAMRQANVDYSELSGIAAAAGPGLIGGVIVGLTTAKAIALAAKKPLVAVNHLE
AHALTARLTDGVAFPYLLLLVSGGHTQLLAIEDVGKYTRLGTTLDDAVGEAFDKVAKMLS
LPYPGGPQVEEQAKHGYSARFAFPRPLLGRREPDFSLSGLKTAVRLEADRLAPLSDEDVA
DLCASFQAAVVDLIADRVRAGARAFRDRLGRGPTALVVAGGVAANGMIRESLAKVAAELN
TRFAMPAPVLCTDNGAMIAWAGAERLARGIRDRLDVSPRARWPLDPAAAASATARA