Protein Info for GFF2088 in Variovorax sp. SCN45

Annotation: Putative lipase in cluster with Phosphatidate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 PF12146: Hydrolase_4" amino acids 34 to 269 (236 residues), 226.4 bits, see alignment E=4.9e-71 PF00561: Abhydrolase_1" amino acids 40 to 145 (106 residues), 38.2 bits, see alignment E=1.9e-13 PF12147: Methyltransf_20" amino acids 277 to 585 (309 residues), 502.3 bits, see alignment E=7.6e-155

Best Hits

Swiss-Prot: 58% identical to YNBC_ECOLI: Uncharacterized protein YnbC (ynbC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to vpe:Varpa_5286)

Predicted SEED Role

"Putative lipase in cluster with Phosphatidate cytidylyltransferase" in subsystem Triacylglycerol metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (588 amino acids)

>GFF2088 Putative lipase in cluster with Phosphatidate cytidylyltransferase (Variovorax sp. SCN45)
MTDITQRVPHEHHFQTHDGESLFYRHWPATGGARRGAILLFHRGHEHGARMAHLVDELDL
PDFDFFAWDARGHGRSPGQRGYSPSFGTSVRDVQTFVQHIGAAHGVPEQDIHVVAQSVGA
VLIATWAHDYAPKVRGLTLASPAFKVKLYVPFARPGLALMHKLRGLFFVNSYVKAKFLTH
DPERIASYESDPLISRPIAVNILLGLYEAADRVVADANAITLPVQLLISGADWVVHHKPQ
HRFFDRLGSAVKTKTELPGFFHDTLGEKDRAPAVAQIREFILQRFDEPAVPVDRREAHLS
GDTADESRALAEPLSPLSPRGAYWAMTRAGLKLGGKMSEGIKLGHDTGFDSGSTLDYVYR
NQPRGSSFIGRSIDKTYLESIGWRGIRQRKIHVEELLRIAMERLAEMHREVRVMDIAAGH
GRYVLDAVLASPVKASSILLRDYSDINVRDGRALIAEKGLEDVAQFVQADAFDRMSLASV
TPRPTLAVVSGLYELFPDNEMVRRSLAGVGDAVEDRGYLVYTGQPWHPQLEMIARALTSH
RQGEAWVMRRRTQFEMDQLVEEAGFRKIDQRVDEWGIFTVSLAVRTER