Protein Info for PS417_10645 in Pseudomonas simiae WCS417

Annotation: magnesium ABC transporter ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 901 transmembrane" amino acids 103 to 121 (19 residues), see Phobius details amino acids 127 to 144 (18 residues), see Phobius details amino acids 242 to 261 (20 residues), see Phobius details amino acids 291 to 312 (22 residues), see Phobius details amino acids 320 to 345 (26 residues), see Phobius details amino acids 710 to 735 (26 residues), see Phobius details amino acids 771 to 793 (23 residues), see Phobius details amino acids 806 to 824 (19 residues), see Phobius details amino acids 836 to 857 (22 residues), see Phobius details amino acids 869 to 889 (21 residues), see Phobius details TIGR01524: magnesium-translocating P-type ATPase" amino acids 45 to 901 (857 residues), 1390.8 bits, see alignment E=0 PF00690: Cation_ATPase_N" amino acids 56 to 119 (64 residues), 47.9 bits, see alignment 2.2e-16 TIGR01494: HAD ATPase, P-type, family IC" amino acids 130 to 410 (281 residues), 123.6 bits, see alignment E=7.6e-40 amino acids 612 to 732 (121 residues), 102 bits, see alignment E=2.7e-33 PF00122: E1-E2_ATPase" amino acids 159 to 353 (195 residues), 146.3 bits, see alignment E=1.8e-46 PF00702: Hydrolase" amino acids 370 to 656 (287 residues), 61 bits, see alignment E=5.2e-20 PF13246: Cation_ATPase" amino acids 437 to 483 (47 residues), 37 bits, see alignment 7.3e-13 PF00689: Cation_ATPase_C" amino acids 725 to 891 (167 residues), 67.4 bits, see alignment E=3.5e-22

Best Hits

Swiss-Prot: 66% identical to ATMA_ECO57: Magnesium-transporting ATPase, P-type 1 (mgtA) from Escherichia coli O157:H7

KEGG orthology group: K01531, Mg2+-importing ATPase [EC: 3.6.3.2] (inferred from 76% identity to adk:Alide2_0107)

Predicted SEED Role

"Mg(2+) transport ATPase, P-type (EC 3.6.3.2)" (EC 3.6.3.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.2

Use Curated BLAST to search for 3.6.3.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U2F6 at UniProt or InterPro

Protein Sequence (901 amino acids)

>PS417_10645 magnesium ABC transporter ATPase (Pseudomonas simiae WCS417)
MNLTLLKEFFAGFLRTRHIARHFRRLAMLDSVTDASVSREVPPTLAQTLVVSANSSAVQL
LGTLGSHSEGLNTQEADALRVQYGLNEVEHEQPLPGWVHLWHCYKNPFNLLLTLLAVISW
LTEDMKAATVIFSMVVLSTLLRFWQEAKSNKAADALKAMVSNTATVLRRDEAKRIELPIK
QLVPGDLIVLSAGDMIPADCRVLSAKDLFVSQAAMTGESMPVEKFAQQQDTKTRNPLDLE
NILFMGTNVVSGAATAVILTTGNSTYFGALAQRVTATDRATTSFQHGVNKVSWLLIRFMF
VMAPLVLFINGFTKGDWTEALLFALSIAVGLTPEMLPMIVTSTLAKGAVFLSRKKVIVKR
LDAIQNFGAMDVLCTDKTGTLTQDKIFLARHVDVWGEESDDVLEMAYLNSYYQTGLKNLL
DVAVLEHVEVHRELKVGTAFQKVDEIPFDFNRRRMSVVVAEQGQPHLLICKGAVEEILSV
CNNVRHGDVNEALTEDLLARIRQVTAAFNEEGLRVVAVAAQPMAPGRDTYSLADENNLTL
IGYVAFLDPPKESTAPALKALKAHGVAVKVLTGDNELVTAKICREVGLEQQGLLMGNDIE
AMTDAELAKAVETTNVFAKLTPSHKERIVRLLKANGHVVGFMGDGINDAPALRTADIGIS
VDSAVDIAKEAADIILLEKSLMILEEGVLEGRRTFANMLKYIKMTASSNFGNVFSVLVAS
AFIPFLPMLPMHLLVQNLLYDISQIAIPFDNVDAQMLSKPQRWQPGDVGRFMLFFGPISS
IFDITTFALMWYVFDANTPDHQTLFQSGWFVVGLLTQTLIVHMIRTPKIPFLQSRAAMPL
MVMTGVIMAVGIFLPMGPLAHYFKLQALPSLYFVFLPVILLAYMALTQAVKGYYIRKFGW
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