Protein Info for GFF2087 in Methylophilus sp. DMC18

Annotation: Cyclic di-GMP phosphodiesterase CdgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 PF00563: EAL" amino acids 69 to 195 (127 residues), 33.1 bits, see alignment E=3.8e-12 PF08668: HDOD" amino acids 217 to 322 (106 residues), 37.5 bits, see alignment E=1.8e-13

Best Hits

KEGG orthology group: K07181, putative signal transduction protein containing EAL and modified HD-GYP domains (inferred from 58% identity to mmb:Mmol_1564)

Predicted SEED Role

"Predicted signal transduction protein" in subsystem Flagellar motility

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (406 amino acids)

>GFF2087 Cyclic di-GMP phosphodiesterase CdgJ (Methylophilus sp. DMC18)
MTTSQHSIIGRQPVVNDQQQIIGYEFFFRNETDGSNTPFEEDIQTCARILNTTMDEMHES
WLLGQQLAFINVDHVMLNSVFLELMPAEKTVLEVVHHLQVTPEILARCQELKQLKFRIAL
DNPQHYPHLEALLPYADFIKIDMRNVDIAQAKQWLKKYQHDQCSLIAEKVEENNQFQDLL
DAGYHQFQGYFYAKPENISSKVINPSFDGVLHLLNLVSQEAENKDIENGFKRDTTLSYKL
LRYINSVGFGLSCEVQSIGHALTILGRNQLYRWLTLLMVTAGNNSSSPALMKTSITRGRL
TELLGEPFFEKRDRDNLFVVGVFSLLDVMLKVPMENILEKLQLPETIVDALVERQGIFGP
FLKLTEACEDANNEEILEMAAMLQLKPEKVNQCHMEALAWTEALGI