Protein Info for PS417_10550 in Pseudomonas simiae WCS417

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 TIGR01056: DNA topoisomerase III" amino acids 1 to 637 (637 residues), 700.2 bits, see alignment E=1.7e-214 PF01751: Toprim" amino acids 3 to 129 (127 residues), 58.3 bits, see alignment E=6.9e-20 PF01131: Topoisom_bac" amino acids 149 to 589 (441 residues), 386.5 bits, see alignment E=1.7e-119

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 97% identity to pfs:PFLU2157)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U830 at UniProt or InterPro

Protein Sequence (649 amino acids)

>PS417_10550 DNA topoisomerase III (Pseudomonas simiae WCS417)
MRLYLCEKPSQAKDIAAVLGASRRGDGCWLGNGVTVTWCIGHLLETAPPDAYDEKYKRWV
LADLPIVPEKWKMRVKPKTASQFKAVKRLLGEAQELVIATDADREGEMIARELVEHCRYR
GPIQRLWLSALDDASIRKALAALKPGAETFSLYHSALGRSRADWLIGMNMSRLFTLLGRQ
SGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVDLQHERITFTAQWRAAEDAC
DDQGRCLDPQLARDAADAMRNAATARLVKLRTERMREVAPLPFDLGTLQEICSKKLGLGA
QETLDIAQSLYETHKVITYPRSDCGYLPLSQHSEAPKILAALARADAAVNELMPHIETQR
RSRAWNDAKVSAHHGIIPTGAGKDVGQLTGKHRAVYTLIRARYLAQFLPNHEYDRTQADF
DCAGQALRAVGKVVIEPGWKRALPEALAPAKGREAPAPQALPALVQGQDYAVAKVNLKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLVK
NGKALSATPAAFSLIDAVPRAIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQV
ARCNGMRMTITGPASPAGRGATPWKKKRKSAPRKTAASPKRAKKPTNAG