Protein Info for GFF2066 in Variovorax sp. SCN45

Annotation: Tyrosyl-tRNA synthetase (EC 6.1.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 TIGR00234: tyrosine--tRNA ligase" amino acids 11 to 400 (390 residues), 387.8 bits, see alignment E=3.3e-120 PF00579: tRNA-synt_1b" amino acids 39 to 320 (282 residues), 274.2 bits, see alignment E=1.3e-85

Best Hits

Swiss-Prot: 78% identical to SYY_CUPNJ: Tyrosine--tRNA ligase (tyrS) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 96% identity to vpe:Varpa_5300)

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (402 amino acids)

>GFF2066 Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Variovorax sp. SCN45)
MSLSNSVGRALEISLRGADELLPQDEWVRKLQRSEATGTPLRIKLGLDPTAPDIHIGHTV
VLNKMRQLQDLGHRVIFLIGDFTSTIGDPSGRNNTRPPLTREQIEANAQTYYKQASLVLD
PDKTEIRYNSEWSDPLGARGMIQLAAKYTVARMMERDDFNKRFKAGQSISVHEFLYPLMQ
GYDSVALKSDLELGGTDQKFNLLVGRHLQQEYGQEPQCILTMPLLEGLDGVEKMSKSKNN
YIGISEDANTMFAKVLSISDDLMWRWYTLLSFQSLEQIAALKAEIEGGRNPKDAKVALAK
EITTRFHSAAAADTAEQDFINRSKGGVPDEIPEVALSGAPLGIAQLLKQANLAPSTSEGN
RLIDGGGVRIDSVVVSDKALKLPAGSYVVQVGKRKFARVTLS