Protein Info for GFF2063 in Variovorax sp. SCN45

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 16 to 38 (23 residues), see Phobius details amino acids 54 to 74 (21 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details amino acids 140 to 162 (23 residues), see Phobius details amino acids 168 to 189 (22 residues), see Phobius details amino acids 203 to 220 (18 residues), see Phobius details amino acids 228 to 246 (19 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details amino acids 296 to 316 (21 residues), see Phobius details amino acids 332 to 350 (19 residues), see Phobius details amino acids 357 to 377 (21 residues), see Phobius details amino acids 397 to 422 (26 residues), see Phobius details amino acids 434 to 455 (22 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 19 to 431 (413 residues), 240 bits, see alignment E=2.5e-75 PF07690: MFS_1" amino acids 21 to 408 (388 residues), 161.4 bits, see alignment E=2.9e-51 PF00083: Sugar_tr" amino acids 27 to 116 (90 residues), 26.4 bits, see alignment E=3.2e-10

Best Hits

Swiss-Prot: 51% identical to MDTD_CROS8: Putative multidrug resistance protein MdtD (mdtD) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: None (inferred from 94% identity to vpe:Varpa_5303)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>GFF2063 Uncharacterized MFS-type transporter (Variovorax sp. SCN45)
MTSEAETAVQPVRKSLLWLVAVGFFMQTLDATIINTALPAMAASLGESPLRMQSVVVAYA
LTMAMLIPASGWIADRFGTRRIFFSAIVLFALGSVLCALSHSLGQLVAARVVQGLGGALL
LPVGRLSLLRTVPRGEFLQAMSFVAIPGLIGPLLGPTLGGWLVQYASWHWIFLINVPVGL
AGCLATLKYMPDLRGVVQKRFDSVGYAMLAFGMVAISLSLDGVGLRQGGVMVVLIFGFAS
IAAYWLRASRTPEPLFAPSLFHVPTLSIGLIGNLFSRLGSSCMPFLVPLLLQVSMGYSPV
RAGLMMLPIALAGMAMKRFATPLITRHGYRRVLVVNTALVGCTMASFGLTAPGQPMVLHV
LQLLAFGAVNSLQFTAMNTITLKDLDGSMASSGNSLLSMVQMLAMSMGVAAAGAVLAGYN
GIFGTETAAHTLDAFQATFATMGLITVASALIFWHLPSEVRAAHPAQPEVSGQG