Protein Info for GFF205 in Xanthobacter sp. DMC5

Annotation: 3-oxoadipate enol-lactonase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02427: 3-oxoadipate enol-lactonase
  • amino acids 15 to 256 (242 residues), 273 bits, E=1.1e-85
• PF00561: Abhydrolase_1
  • amino acids 22 to 244 (223 residues), 66.9 bits, E=3.3e-22
• PF12697: Abhydrolase_6
  • amino acids 24 to 250 (227 residues), 54.3 bits, E=4.6e-18
• PF12146: Hydrolase_4
  • amino acids 46 to 227 (182 residues), 34.5 bits, E=2.1e-12

Best Hits

KEGG orthology group: K01055, 3-oxoadipate enol-lactonase [EC: 3.1.1.24] (inferred from 84% identity to xau:Xaut_3475)

MetaCyc: 40% identical to subunit of 3-oxoadipate enol-lactone hydrolase (Pseudomonas putida)
3-oxoadipate enol-lactonase. [EC: 3.1.1.24]

Predicted SEED Role

"Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)" in subsystem Catechol branch of beta-ketoadipate pathway or Chloroaromatic degradation pathway or Protocatechuate branch of beta-ketoadipate pathway (EC 3.1.1.24)

MetaCyc Pathways

• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• catechol degradation to β-ketoadipate (4/4 steps found)
• aromatic compounds degradation via β-ketoadipate (7/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (2/4 steps found)
• 4-methylcatechol degradation (ortho cleavage) (4/7 steps found)
• toluene degradation III (aerobic) (via p-cresol) (5/11 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• superpathway of aerobic toluene degradation (9/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (11/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.24

Use Curated BLAST to search for 3.1.1.24

Protein Sequence (257 amino acids)

>GFF205 3-oxoadipate enol-lactonase 2 (Xanthobacter sp. DMC5)
VALLEVSGERFRVEMTGPEGAPPLILSHSLGCTLGMWDEVVPLFAGRRLIRYDARGHGES
AAPDQVYSMGDLGRDVLAIMDRLHLATADFCGLSLGGMVGQWLALNAPQRLTRLILSNTT
AHAGPARFWDHRIKAVRKDGTGPIADSVIDSWFTPELKAQRPARVAEVRAMVTGTKPAGY
MGTSAAMRDMDFRTDVARITTQTLVIVSDGDRSTPPAWGEAIAAAIPGGKLARLPGGHLS
AIEQPEAFARTVSAFLG