Protein Info for GFF204 in Methylophilus sp. DMC18

Annotation: Nitrate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 51 to 71 (21 residues), see Phobius details amino acids 79 to 99 (21 residues), see Phobius details amino acids 105 to 126 (22 residues), see Phobius details amino acids 138 to 162 (25 residues), see Phobius details amino acids 168 to 187 (20 residues), see Phobius details amino acids 211 to 236 (26 residues), see Phobius details amino acids 247 to 268 (22 residues), see Phobius details amino acids 278 to 297 (20 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 332 to 352 (21 residues), see Phobius details amino acids 363 to 382 (20 residues), see Phobius details PF07690: MFS_1" amino acids 23 to 348 (326 residues), 151.4 bits, see alignment E=3.3e-48 PF13347: MFS_2" amino acids 42 to 318 (277 residues), 27.3 bits, see alignment E=1.4e-10

Best Hits

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 86% identity to mei:Msip34_0347)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>GFF204 Nitrate transporter (Methylophilus sp. DMC18)
MQKSFWEVGHKPTLFSAFFYFDLSFMVWVMLGPLAVQIAADLQLNPAQKGLMVATPVLTG
ALLRIVMGVLVDQLKPKKAGLIGQVIVIGALLTAWLHGIHTYQQALLLGMFLGFAGAAFA
VALPLASRWYPPEHQGTALGIAGAGNSGTALAALFAPGLAVAFGWNNVFGLALIPLALAL
LVYVTMAKDAPDTPAPKSLSQYLAVLKDKDAWWFMFFYSVTFGGFVGMASSLTIYFHDQY
GLDAVHAGYFTAACVFAGSLVRPIGGWLADRIGGIRSLSTMYVLAAVFLFILSFGMASQS
LGLLVVVLAMAALGMGNGAVFQLVPQRFRKEIGVMTGLVGMAGGVGGFYLASSLGYSKQV
TGSYQVGLTIFAGLAILALIGLSRVKNRWRTTWGAATVTSAKI