Protein Info for GFF2016 in Methylophilus sp. DMC18

Annotation: Thiamine thiazole synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00743: FMO-like" amino acids 2 to 427 (426 residues), 136.8 bits, see alignment E=4.2e-43 PF00070: Pyr_redox" amino acids 2 to 33 (32 residues), 24.5 bits, see alignment (E = 1.5e-08) PF07992: Pyr_redox_2" amino acids 2 to 202 (201 residues), 58.7 bits, see alignment E=3.4e-19 PF01266: DAO" amino acids 3 to 44 (42 residues), 30 bits, see alignment 2.1e-10 PF13454: NAD_binding_9" amino acids 4 to 77 (74 residues), 24.5 bits, see alignment E=1.2e-08 PF13450: NAD_binding_8" amino acids 5 to 47 (43 residues), 38.4 bits, see alignment 6e-13 PF13738: Pyr_redox_3" amino acids 5 to 208 (204 residues), 66.2 bits, see alignment E=1.6e-21 PF01593: Amino_oxidase" amino acids 10 to 40 (31 residues), 22.5 bits, see alignment (E = 3.2e-08) PF13434: Lys_Orn_oxgnase" amino acids 90 to 199 (110 residues), 22.8 bits, see alignment E=2.4e-08

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (504 amino acids)

>GFF2016 Thiamine thiazole synthase (Methylophilus sp. DMC18)
MNIAIVGAGFAGLSTAKVLTAFGHQVTVFEKEADVGGVWSASRRYPNLTTQNVKSTYAFS
DFPYPKHYPEWPSGEQVQHYLDAYTKQFNFAHLISLNTEVKLATPVGQGWQLTVKTEGKA
EETRHFDFLIVCNGIFSQPMIPDYPGAAEFTAAGGRICHTSEFKELADAKGKHVLIVGYG
KSSTDVAQAVCQQAASTTVVARHLIWKVPKRLMNVLNYKYLFLTRMGEGLFKYIRLKGIE
RFLHGIGKPIRNSMLNQVQWVINRQCRLEKLGLKPDQPLESIARSTVSLVTEGFFESVEA
GHIDVAKQNSITGFSIEHGVRYAHLTDGRRLPADIVICGTGWHQRVPFLNDDVMKHVTDA
EGNFVLYRSMAPVGMPGLAFNGYNSSFFSQLNAEVGAYWLADWIGGKMKLPTSEAQRSTI
KERLAWMEARTEGKHSKGTNIIPFSLHHIDELLSDMRLPLGVATRFKQWFLPIDPADFQY
LHKALLQRHQPNAAQKMPVQTKTV