Protein Info for GFF2007 in Sphingobium sp. HT1-2

Annotation: Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 PF01590: GAF" amino acids 28 to 157 (130 residues), 60.8 bits, see alignment E=5.4e-20 PF13185: GAF_2" amino acids 30 to 160 (131 residues), 51 bits, see alignment E=4.4e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 185 to 738 (554 residues), 387.4 bits, see alignment E=5.1e-120 PF05524: PEP-utilisers_N" amino acids 185 to 303 (119 residues), 75.8 bits, see alignment E=8.5e-25 PF00391: PEP-utilizers" amino acids 331 to 401 (71 residues), 56.1 bits, see alignment E=5.7e-19 PF02896: PEP-utilizers_C" amino acids 429 to 722 (294 residues), 304 bits, see alignment E=2.6e-94

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 90% identity to sch:Sphch_1602)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (757 amino acids)

>GFF2007 Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated (Sphingobium sp. HT1-2)
MPSTPAAAARQILVRLQEVMAARTSAQAKLNKVVEIIGESLSSEVCSIYLVREGVLELFA
TRGLKQEAVHVTRMAKGEGLVGLIATNVEPLNLDEAAAHPDFSYRPETGEEMFHSFAGVP
IVRRERALGVLCVQHVEPRKYEEVEIEALQTVAMVLSELIANAELADDGPTDARVQETGS
NIMTGLQLVMGMARGHVVFHQPRVHIEHTVAEDTEAERQRVISAFAKMREQIDRMTGTAD
FGTEGEHQEVLETYKMFAYDEGWIRRINEAIDSGLTAEAAIERVQQRTRMRMRQIDDPLL
QDRMHDLEDMANRLLRIVSGQLGTAAQLGLRQDAILIARNLGPAELLEYDRRRLKGVILE
EGSLTAHVTIVARAMGVPVLGRVRDIRHRVNEGDLILMDVAENALLIRPTPDMEEAFETK
LHITQKRRAEFAALRDLPSVTQDGARIELMVNAGLREDAQALDMVGADGIGLFRTEFQFL
VSATLPQREKQQRLYKDVLDAAGDRPVIFRTVDIGGDKALPYMTREDDEHEDNPAMGWRA
IRLALDRDGLMKAQARALLEASAGKVLNIMFPMVSEPWEFEEARTLVEHQRQWLIGQKKK
LPVAVRYGAMLEVPALAEVLDLLLPQLDFLSIGTNDLTQFLFAADRAHPKLAERYDWLSL
AILRFLDRVVRICEIYKVPVGVCGEMGGRTLEAMALIGLGVRRLSITPASVGPVKAMIRA
IDAAELQALMRDLLDSGASDLRAQLTSWAEQRNIELA