Protein Info for GFF200 in Variovorax sp. SCN45
Annotation: Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to NAGG_RALSP: Salicylate 5-hydroxylase, large oxygenase component (nagG) from Ralstonia sp.
KEGG orthology group: K05708, large terminal subunit of phenylpropionate dioxygenase [EC: 1.14.12.19] (inferred from 94% identity to vpe:Varpa_2933)MetaCyc: 80% identical to salicylate-5-hydroxylase large subunit (Ralstonia sp. U2)
RXN-10446 [EC: 1.14.13.172]
Predicted SEED Role
"Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB" in subsystem Benzoate degradation
MetaCyc Pathways
- salicylate degradation II (1/1 steps found)
- salicylate glucosides biosynthesis II (1/2 steps found)
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (2/5 steps found)
- cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (2/5 steps found)
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (2/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.14.12.19
Use Curated BLAST to search for 1.14.12.19 or 1.14.13.172
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (418 amino acids)
>GFF200 Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB (Variovorax sp. SCN45) MTTQAVFPVELKWESEQTGRIPFMAYTDEALHKKELQRFFYEKHWCYVGLEAEIPNPGDF KRTAIGERSVIMSRDEAGEIHVFENVCAHRGMQFCRERHGNKKEFVCPYHQWNYTLKGDL QGVPFRRGVKQDGKVNGGMPSDFKTEENGLTKLKVASRGGVVFASFDHEIESFEDFLGPD ILHYFDRLFDGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKS ELKMDPRGRHAAMISTRGSAGKAAQVTQVSSFKESMQLKDPRFLDIVPEPWWGGPTAVMM TLFPSLILQQQVNSVSTRHIQPVGHDAFDFVWTHFGFEDDTEEMTQRRLRQANLFGPAGF VSADDGEVIEFSQEGFEQKPYHRTLAELGGKEVANTDHMVTETLIRGMYRYWREVMEA