Protein Info for GFF1994 in Variovorax sp. SCN45

Annotation: Glutamate carboxypeptidase (EC 3.4.17.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF04389: Peptidase_M28" amino acids 95 to 212 (118 residues), 47.8 bits, see alignment E=2.3e-16 PF01546: Peptidase_M20" amino acids 109 to 410 (302 residues), 104.9 bits, see alignment E=8.3e-34 PF07687: M20_dimer" amino acids 213 to 312 (100 residues), 71.3 bits, see alignment E=9.1e-24

Best Hits

Swiss-Prot: 97% identical to CBPG_PSES6: Carboxypeptidase G2 (cpg2) from Pseudomonas sp. (strain RS-16)

KEGG orthology group: None (inferred from 96% identity to vpe:Varpa_5372)

Predicted SEED Role

"Glutamate carboxypeptidase (EC 3.4.17.11)" (EC 3.4.17.11)

Isozymes

Compare fitness of predicted isozymes for: 3.4.17.11

Use Curated BLAST to search for 3.4.17.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>GFF1994 Glutamate carboxypeptidase (EC 3.4.17.11) (Variovorax sp. SCN45)
MRPSIHRTALAALLATAFLAPAASWAQKRDNVLFQAATDEQPAVIKTLEKLVNIETGTGD
AEGIAAAGNYLEGELKNLGFTVTRSKSAGIVVGDNIVGKIKGRGGKNLLLLSHMDTVYLK
GILAKAPFRVEGDKAYGPGIADDKGGNAVILHTLKLLKDYGVRDYGTITVLFNTDEEKGS
FGSRDLIQEEAKLADYVLSFEPTSAGDEKLSLGTSGIAYVQVNITGKASHAGAAPELGVN
ALVEASDLVLRTMNIDDKSKNLRFNWTIAKAGNVSNIIPATATLNADVRYARNEDFDAAM
KTLEERAQQKKLPEADVKVIVTRGRPAFNAGEGGKKLVDKAVAYYKEAGGTLGVEERTGG
GTDAAYAALSGKPVIESLGLPGFGYHSDKAEYVDISAIPRRLYMAARLIMDLGAGK