Protein Info for GFF1992 in Variovorax sp. SCN45

Annotation: Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 PF20423: AceK_regulatory" amino acids 15 to 315 (301 residues), 400.6 bits, see alignment E=4.2e-124 PF06315: AceK_kinase" amino acids 317 to 593 (277 residues), 388.1 bits, see alignment E=2e-120

Best Hits

Swiss-Prot: 78% identical to ACEK_ACIAC: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 96% identity to vpe:Varpa_5374)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (606 amino acids)

>GFF1992 Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-) (Variovorax sp. SCN45)
MFPQRLDSPLAYDVAKAMMDGFNRHYRLFRAESARAKHRFETADWHGQQRAQRERIEFYD
LRVSEAVARLEKEFKAGDQPMDVWHQVKLHYIGLLVDHHQPELAESFFNSVTTKILHRAY
FQNDFIFVRPAISTEYIEPRGAPTYRPYYPSPDTLADTVEQMLDDFSLLGSFADKRRDAE
RVAHVILNRFHQVKLRANFQLQVLSGLFFRNKGAYVVGKIMNGFAEIPFSLPILHDSRGR
FYIDAALFGEDDLQMLFSFARAYFMVDMEVPSAWVQFLRSLMPRKPRAEIYNALGLAKQG
KTLFYRDFLSHLNYSSDRFRIAPGIKGMVMLVFDLPSFPFVFKVIKDFYPPQKDTTREQI
KGKYMLVKQHDRVGRMADTLEYSDVGFPLDRFEPGLIEEIRKFAPSQLEIGDRDGNGEME
LVVKHVYIERRMIPLNIYLQEAFDTLAHPESPAHARRAKDQLEHAVVEYGNAIKDMVAAN
IFPGDMLWKNFGVTRGGKVVFYDYDEIEYVTDCKFRKVPTPRNEEEEMSGEVWYSVGPKD
VFPETFEPFLLGNKDVREAFMAHHADLLDAAFWQHHKERIQAGQMLDVFPYEESQRFQHE
GHATHF