Protein Info for GFF1980 in Variovorax sp. SCN45

Annotation: Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 transmembrane" amino acids 588 to 607 (20 residues), see Phobius details TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 5 to 712 (708 residues), 625.1 bits, see alignment E=1.1e-191 PF02092: tRNA_synt_2f" amino acids 10 to 576 (567 residues), 625.2 bits, see alignment E=1.2e-191 PF05746: DALR_1" amino acids 606 to 703 (98 residues), 32.3 bits, see alignment E=9.1e-12

Best Hits

Swiss-Prot: 78% identical to SYGB_POLNA: Glycine--tRNA ligase beta subunit (glyS) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 96% identity to vpe:Varpa_5393)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (714 amino acids)

>GFF1980 Glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Variovorax sp. SCN45)
MTTPMTKNPLLVELFVEELPPKALKKLGDAFAGVLRDQLVAQGLADAGSVLTPYASPRRL
AAHLTHVAERAADKAVSQKLMPVAVGLDASGQPTPALLKRLGALGADASAVPGLKRAMDG
KAEALFYESTAKGATLAEGLQKALAEAIAKLPIPKVMSYQLESGCELPGWSSVSFVRPAH
GLVALHGDTVVPIEALGLKAGRDTHGHRFEAAVDPVVLRNADSYALQLQEEGAVIAGFEA
RRAEIARQLAAAAVKVGGGSVPIHDDALLDEVTALVERPNVLVCSFEREFLEVPQECLIL
TMKANQKYFPLLDASSKLTNKFLVVSNISPADASAVIEGNERVVRPRLADAKFFFDQDRK
KSLASRVESLGKVVYHNKLGTQGERVERVMRIARGIAQKLGDSALAAQATQAAQLAKADL
VTDMVGEFPELQGTMGRYYALHDGLDVAVADAIEDHYKPRFAGDELPRNSVGLVVALADK
LETLVGMFGIGNLPTGDRDPFALRRHALGVIRMLIEKDLPLGLDALLQDAAAQFKDIPGF
ESSKATVELQDFILDRLAGSLREQGASAQEVDAVLAPRPQRLGEVPKLLAAVRAFAALPA
AAALAAANKRIGNILKKAPEADAHVSELLLQEPAEKALHAAMAQVVPAANAQFEAGDYTA
SLQTLAALREPVDAFFDGVMVNAEQADLRLNRLGLLMSLHAAMNRVAQLERLAV