Protein Info for GFF1960 in Variovorax sp. SCN45

Annotation: Phenylalanine-4-hydroxylase (EC 1.14.16.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR01267: phenylalanine-4-hydroxylase" amino acids 35 to 273 (239 residues), 331.9 bits, see alignment E=1e-103 PF00351: Biopterin_H" amino acids 44 to 257 (214 residues), 172.5 bits, see alignment E=6.1e-55

Best Hits

Swiss-Prot: 58% identical to PH4H_RALSO: Phenylalanine-4-hydroxylase (phhA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00500, phenylalanine-4-hydroxylase [EC: 1.14.16.1] (inferred from 92% identity to vap:Vapar_4768)

MetaCyc: 58% identical to phenylalanine hydroxylase (Chromobacterium violaceum)
Phenylalanine 4-monooxygenase. [EC: 1.14.16.1]

Predicted SEED Role

"Phenylalanine-4-hydroxylase (EC 1.14.16.1)" in subsystem Aromatic amino acid degradation or Pterin biosynthesis (EC 1.14.16.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.16.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>GFF1960 Phenylalanine-4-hydroxylase (EC 1.14.16.1) (Variovorax sp. SCN45)
MDSPAAAAVVTPAVYGASDRPPRGDYSRGGAVLADYTCPQDWASYTPADHDTYKRLYERQ
AAQLPGLACDAFIKALPSLGVKDRIPRFDELNERLHRATGWEIVAVPGLIPELPFFTLLA
NRKFPVTDWIRKPEEFDYIVEPDVFHDLFGHVPMLFDPTFADYVQRYGAGGIKAHELGAG
EKLARLYWYTVEFGLIRQPDGLRAYGAGILSSVGELKHAVLSDEPRRLPIDLLRAMRTRY
KIDTYQANYFVIDSFAQLFDLTAPDFTPLYESLEVAPDIEAGVLLPGESGKA