Protein Info for GFF196 in Sphingobium sp. HT1-2

Annotation: Conjugative transfer protein TrbI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 transmembrane" amino acids 33 to 54 (22 residues), see Phobius details PF03743: TrbI" amino acids 207 to 381 (175 residues), 202.7 bits, see alignment E=2.5e-64

Best Hits

KEGG orthology group: K03195, type IV secretion system protein VirB10 (inferred from 43% identity to nwi:Nwi_0860)

Predicted SEED Role

"Conjugative transfer protein TrbI" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (395 amino acids)

>GFF196 Conjugative transfer protein TrbI (Sphingobium sp. HT1-2)
MTEAPEAPAPEKVDPETLALRSRPTRAIRFRRGAIIGIAAAGSASLMAIAWLALKPQVLH
RQAPDSELSQPVAGSASDALAGLPSSYGDAPKLGPPLPGDLGRPILRAQQRVQDAGTPAP
ADDAEARRQQRLAQIQAARESGLLVQVAAGPGSPSGTPRTSTEGSTENPATPAETSATRK
ERFAKARDDGPDFNPHGLVPPVSPNMLLAGSVIAASFITGLNSDLPGMVTAQVTQNAYDT
VTGRILLIPQGSRLVGKYDSVVAFGQKRALVVWQRLILPDGSSVRLDNMPATDPSGYAGL
SDKVDFHTWSLLKGVAIATLLGVGSELSIDGESDLVEAMRQSAQTSTSRAGDQITQRNLD
VQPTITVRPGAPVRVLVTRDLLIESGDRRGKPWSS