Protein Info for GFF195 in Sphingobium sp. HT1-2

Annotation: Conjugative transfer protein TrbG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR02775: P-type conjugative transfer protein TrbG" amino acids 46 to 253 (208 residues), 258.4 bits, see alignment E=2.1e-81 PF03524: CagX" amino acids 47 to 264 (218 residues), 222 bits, see alignment E=3.4e-70

Best Hits

KEGG orthology group: K03204, type IV secretion system protein VirB9 (inferred from 59% identity to sjp:SJA_C1-29630)

Predicted SEED Role

"Conjugative transfer protein TrbG" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (274 amino acids)

>GFF195 Conjugative transfer protein TrbG (Sphingobium sp. HT1-2)
MKQAILPALAGLACAGSAHAQSTIQQVAAANRAAIVQPTAGGFVAGAQVYPYREDAIFES
YVSPGLVTDLALQPGEGLVAIASGDTARWVIGDTISGAGDARQVHVLVKPFAPGLATNLV
ITTDRRTYHIKLVSTPGTAISAVRWTYPQDELLALKRKADAAQAAAPVAAGLSIEQLHFN
YHIAGDRPAWRPLRAFDDGSRTYIEFSASLAQGEAPPLFLVGADGKAELVNYRLRDRYYV
VDRIFAAAELRLGLKKQQVVRITRVSPPANGSGR