Protein Info for GFF1945 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 transmembrane" amino acids 267 to 284 (18 residues), see Phobius details PF05430: Methyltransf_30" amino acids 118 to 244 (127 residues), 167.1 bits, see alignment E=1.5e-53 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 267 to 662 (396 residues), 536.6 bits, see alignment E=2e-165 PF01266: DAO" amino acids 268 to 632 (365 residues), 182.8 bits, see alignment E=1.4e-57

Best Hits

Swiss-Prot: 100% identical to MNMC_SALTY: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 82% identity to cko:CKO_00461)

MetaCyc: 80% identical to fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase (Escherichia coli K-12 substr. MG1655)
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase. [EC: 2.1.1.61]; RXN0-7081 [EC: 2.1.1.61]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (666 amino acids)

>GFF1945 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
VKQYAIQPATLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLAERFPVHSHPLF
IVAESGFGTGLNFLTLWQAFDSFRSAHPQATLQRLHFISFEKFPLTRDDLALAHQHWPEL
APWAEQLQAQWPLPLPGCHRLLLDRGRVTLDLWFGDINELTDQLDATLNQTVDAWFLDGF
APAKNPDMWTPNLFNAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQHLMPTLSAPWFYRSGSEKRETAIIGGGIASALLSLALLRRGWQVTLYCADDQPA
QGASGNRQGALYPLLSKHDAAINRFFPTAFTFARRLYDALPVSFDHDWCGVTQLGWDEKS
QQKIAQMLSLALPAGLASALNAEEAEQAVGVTTRCGGITYPAGGWLCPEQLTRAVIALAT
EQGLQTRFRHTLTSLVAQESRWQLRFTSGETASHETVVLANGHQINRFDQTQPLPVYAVG
GQVSHIPTTPALSALRQVLCYDGYLTPQNPHNQQHCIGASYHRGDESTVWREEDQRQNRQ
RLLDCFPDAKWATEVDVSGNSARCGVRCATRDHLPMVGNVPDYHATLTHYADLADNKTSA
APAPVYPGLFMLGALGSRGLCSAPLCAEILAAQMSNEPIPLDAGTLAALNPNRLWVRKLL
KGKAVK