Protein Info for GFF1932 in Methylophilus sp. DMC18

Annotation: Aliphatic amidase expression-regulating protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 49 to 392 (344 residues), 312.9 bits, see alignment E=6.1e-97 TIGR03407: urea ABC transporter, urea binding protein" amino acids 50 to 407 (358 residues), 515.6 bits, see alignment E=3.1e-159 PF13433: Peripla_BP_5" amino acids 50 to 409 (360 residues), 415.3 bits, see alignment E=3.4e-128 PF01094: ANF_receptor" amino acids 72 to 346 (275 residues), 31.1 bits, see alignment E=2e-11

Best Hits

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 91% identity to meh:M301_1358)

Predicted SEED Role

"Urea ABC transporter, urea binding protein" in subsystem Urea decomposition

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (412 amino acids)

>GFF1932 Aliphatic amidase expression-regulating protein (Methylophilus sp. DMC18)
MSTSNRRGFMKGALVGAMMAAGLISGHAFAADYPTAKVNTTGLAVTDSTVKVGILHSATG
TMAISETGSIQAEKLAIEQINAMGGILGRKIEIIQEDGASDWPTFAEKAKKLLEKDKVAA
VMGCWTSASRKAALPVFEKDNGLLFYPTFYEGLEQSKNVFYTGQEATQQILAGLDWIAKE
KKAKTFYLIGSDYIWPRTSMKIARKHIEQHLHGTVVGEEYIALGDTQFGSVINKIKLKKP
DVIYAAVVGGSNVAWFKQLNAAGLNSSKQTMLTISVTEDEVLGIGGENLAGFYSAMKYFQ
SQDNEANKKFVEAFKKRWGEKSVIGDVTQAAYLGPWLYKAAVERAGSFDVDKVQAALPGY
TFKDAPEGPVTVEANHHLTTKLRIGQWGKDGQAKVVYTSDYIKPDPFPKGYQ