Protein Info for GFF1928 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 2 to 868 (867 residues), 1189.1 bits, E=0
• PF00809: Pterin_bind
  • amino acids 21 to 259 (239 residues), 218.5 bits, E=2.3e-68
• PF02607: B12-binding_2
  • amino acids 318 to 394 (77 residues), 80.6 bits, E=1.5e-26
• PF02310: B12-binding
  • amino acids 416 to 510 (95 residues), 62.7 bits, E=5.9e-21
• PF02965: Met_synt_B12
  • amino acids 609 to 883 (275 residues), 395.5 bits, E=2.7e-122

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 81% identity to aaa:Acav_0266)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• folate transformations II (plants) (8/11 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (898 amino acids)

>GFF1928 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLLSGLEPLRVEPGSLFVNVGERTNVTGSKAFARMILEGRFEDALAVARQQVENGAQIID
VNMDEAMLDSKAAMVRFLNLIAGEPDIARVPVMVDSSKWEVIEAGLRCVQGKGVVNSISM
KEGLDEFKRQARLVKRYGAAAVVMAFDEKGQADTYERKVEICERAYRVLVDEVGFPPEDI
IFDPNIFAIATGIEEHNNYAVDFINATRWIKQNLPGAKVSGGVSNVSFSFRGNDPVREAI
HTVFLYHAIKAGMDMGIVNAGMVGVYDELELELRERVEDVVLNRRPDAGERLVEVAESAK
GSAKDDSKKNEWRALPIRERLSHALVRGINDFITEDTEEMWREIEAGGGRPLNVIEGPLM
DGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLLPYIEAEKLALQAAGGDVKPKGKIVI
ATVKGDVHDIGKNIVTVVLQCNNYEVVNMGVMVPCHEILAMAKAEGADIVGLSGLITPSL
EEMQYVAGEMQKDEHFRGKGIPLLIGGATTSRVHTAVKIAPHYDGPVVYVPDASRSVSVA
QGLLSDQAAAYIAEVNADYERVRTQHANKKQVPLWPLERARLNKTPIDWKAYTPQKPKFI
GKRVFKNFDLNEIAKYIDWGPFFQTWDLAGPFPEILKDEVVGEEARRVFSDGKRMLQRLI
EGRWLSASAVVGLYPANTVHDDDIEXYTDESRTSVALTWHGLRQQAEKHEIDGVMRPSRC
LSDFVAPRGAADDYVGMFAVTAGLGVEKKEKQFLDDLDDYSAIMLKALADRMAEALAECM
HHRVRTDLWGYVADEALSNEELIKEKYQGIRPAPGYPACPDHSVKRAMFDVLQCEDIGMG
LTESLAMTPAASVSGFYLAHPQSTYFNVGKIGDDQVDDLAERSAVARNELQRWLAPNL